Isolation of the Pneumocystis carinii dihydrofolate synthase gene and functional complementation in Saccharomyces cerevisiae

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Serval ID
serval:BIB_80E6154B4F79
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation of the Pneumocystis carinii dihydrofolate synthase gene and functional complementation in Saccharomyces cerevisiae
Journal
FEMS Microbiology Letters
Author(s)
Hauser  P. M., Macreadie  I. G.
ISSN
0378-1097 (Print)
Publication state
Published
Issued date
03/2006
Volume
256
Number
2
Pages
244-50
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
The Pneumocystis carinii gene encoding the enzyme dihydrofolate synthase (DHFS), which is involved in the essential biosynthesis of folates, was isolated from clones of the Pneumocystis genome project, and sequenced. The deduced P. carinii DHFS protein shares 38% and 35% identity with DHFS of Schizosaccharomyces pombe and Saccharomyces cerevisiae, respectively. P. carinii DHFS expressed from a plasmid functionally complemented a S. cerevisiae mutant with no DHFS. Comparison of available DHFSs with highly similar folylpolyglutamate synthases allowed the identification of potential signatures responsible for the specificities of these two classes of enzymes. The results open the way to experimentally analyse the structure and function of P. carinii mono-functional enzyme DHFS, to investigate a possible role of DHFS in the resistance to antifolates of P. jirovecii, the species infecting specifically humans, and to develop a new class of antifolates.
Keywords
Amino Acid Motifs Amino Acid Sequence DNA, Fungal/chemistry/genetics Fungal Proteins/chemistry/genetics/physiology *Genetic Complementation Test Molecular Sequence Data Peptide Synthases/*genetics/*physiology Pneumocystis carinii/*enzymology/genetics Saccharomyces cerevisiae/*genetics Schizosaccharomyces/genetics Sequence Alignment Sequence Analysis, DNA
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:30
Last modification date
25/09/2019 7:09
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