Development and characterization of microsatellite markers for diploid populations of the wind-pollinated herb Mercurialis annua.

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License: CC BY 4.0
Serval ID
serval:BIB_80D5511CD13B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Development and characterization of microsatellite markers for diploid populations of the wind-pollinated herb Mercurialis annua.
Journal
BMC Research Notes
Author(s)
Machado A.P., Pannell J.R., Tonnabel J.
ISSN
1756-0500 (Electronic)
ISSN-L
1756-0500
Publication state
Published
Issued date
10/08/2017
Peer-reviewed
Oui
Volume
10
Number
1
Pages
386
Language
english
Abstract
Mercurialis annua is a wind-pollinated annual plant that has long been used as a model for the study of ploidy and sexual-systems evolution. However, no molecular markers are yet available for genetic studies of its diploid populations. Here, we develop and characterize a set of eight polymorphic microsatellite markers for diploid dioecious M. annua.
Following an SSR-enrichment protocol, 13 microsatellite markers were proposed, eight of which yielded successful amplification and polymorphism. We screened the eight microsatellite loci in 100 individuals. The number of alleles per marker ranged from 6 to 12, and observed heterozygosity ranged from 0.57 to 0.76. To estimate potential allele scoring errors, these individuals' offspring were genotyped for the same loci, and error rates were estimated from parentage analyses. Error rates ranged from 0 to 6.8%. Cross-amplification tests were performed for congeneric M. huetti and M. canariensis, with successful amplification for seven and six of the eight loci, respectively. The novel microsatellite markers proposed here will be crucial for a multitude of genetic studies of M. annua and further establish its importance as a model species for addressing ecological and population genetic questions.
Keywords
Diploidy, Euphorbiaceae/genetics, Genetic Markers, Genome, Plant, Microsatellite Repeats/genetics, Phylogeography, Pollination, Wind, Dioecy, Metapopulation dynamics, Microsatellites, Population genetics, Range expansion, Sexual systems
Pubmed
Open Access
Yes
Create date
10/04/2018 20:37
Last modification date
20/08/2019 14:41
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