Connexin26 is regulated in rat urothelium by the scaffold protein IB1/JIP-1.

Details

Serval ID
serval:BIB_801059A2D1A8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Connexin26 is regulated in rat urothelium by the scaffold protein IB1/JIP-1.
Journal
Cell communication & adhesion
Author(s)
Tawadros T., Meda P., Leisinger H.J., Waeber G., Haefliger J.A.
ISSN
1541-9061
Publication state
Published
Issued date
2001
Peer-reviewed
Oui
Volume
8
Number
4-6
Pages
303-6
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
Proper function of the wall of bladder requires gap junctional communication for coordinating the responses of smooth muscle (SMC) and urothelial cells exposed to urine pressure. In the rat bladder, Cx43 is expressed by SMC and urothelial cells, whereas Cx26 expression is restricted to the epithelium. We used a model of bladder outlet obstruction, in which a ligature is placed around the urethra to increase voiding pressure. Increased fluid pressure was associated with increased Cx43 and Cx26 mRNA expression and with the activation of a signaling cascade including the transcription factor c-Jun, which is a component of the AP-1 complex. The signaling pathway of the c-Jun NH2 terminal kinase (JNK) requires the presence of the scaffold protein Islet-Brain1/c-Jun amino-terminal kinase Interacting Protein-1 (IB1/JIP-1). Under stress conditions resulting from urine retention, we have found a reduced content of IB1/JIP-1 in urothelial cells, which in turn induced a drastic increase of JNK and AP-1 binding activities. The stress-induced activation of JNK was prevented by overexpressing IB1/JIP-1, using a viral gene transfer approach, a condition which also resulted in a decrease in Cx26 mRNA. The data show that: 1) mechanical stress of urothelial cells activates in vivo JNK, as a consequence of a regulated expression of IB1/JIP-1 and 2) that urothelial Cx26 may be directly regulated by the AP-1 complex.
Keywords
Adaptor Proteins, Signal Transducing, Adenoviridae, Animals, Cell Communication, Connexin 43, Connexins, DNA-Binding Proteins, Gap Junctions, Gene Expression Regulation, Gene Transfer Techniques, JNK Mitogen-Activated Protein Kinases, Mitogen-Activated Protein Kinases, Nuclear Proteins, Protein Binding, Proto-Oncogene Proteins c-jun, Rats, Signal Transduction, Stress, Mechanical, Trans-Activators, Transcription Factor AP-1, Urethral Obstruction, Urothelium
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:48
Last modification date
20/08/2019 15:40
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