Reduced development of CD4-8-B220+ T cells but normal autoantibody production in lpr/lpr mice lacking major histocompatibility complex class I molecules.

Details

Serval ID
serval:BIB_7F2514ABC1B8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Reduced development of CD4-8-B220+ T cells but normal autoantibody production in lpr/lpr mice lacking major histocompatibility complex class I molecules.
Journal
European journal of immunology
Author(s)
Ohteki T., Iwamoto M., Izui S., MacDonald H.R.
ISSN
0014-2980
Publication state
Published
Issued date
1995
Peer-reviewed
Oui
Volume
25
Number
1
Pages
37-41
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The lpr gene has recently been shown to encode a functional mutation in the Fas receptor, a molecule involved in transducing apoptotic signals. Mice homozygous for the lpr gene develop an autoimmune syndrome accompanied by massive accumulation of double-negative (DN) CD4-8-B220+ T cell receptor-alpha/beta+ cells. In order to investigate the origin of these DN T cells, we derived lpr/lpr mice lacking major histocompatibility complex (MHC) class I molecules by intercrossing them with beta 2-microglobulin (beta 2m)-deficient mice. Interestingly, these lpr beta 2m-/- mice develop 13-fold fewer DNT cells in lymph nodes as compared to lpr/lpr wild-type (lprWT) mice. Analysis of anti-DNA antibodies and rheumatoid factor in serum demonstrates that lpr beta 2m-/- mice produce comparable levels of autoantibodies to lprWT mice. Collectively our data indicate that MHC class I molecules control the development of DN T cells but not autoantibody production in lpr/lpr mice and support the hypothesis that the majority of DN T cells may be derived from cells of the CD8 lineage.
Keywords
Animals, Antigens, CD45, Antigens, Surface/immunology, Autoantibodies/biosynthesis, Cell Differentiation/immunology, Female, Flow Cytometry, Histocompatibility Antigens Class I/immunology, Immunophenotyping, Mice, Mice, Mutant Strains, T-Lymphocytes/immunology, beta 2-Microglobulin/deficiency, beta 2-Microglobulin/immunology
Pubmed
Web of science
Create date
11/02/2010 16:28
Last modification date
20/08/2019 15:40
Usage data