Odorant responses of olfactory sensory neurons expressing the odorant receptor MOR23: a patch clamp analysis in gene-targeted mice.
Details
Serval ID
serval:BIB_7F041E2ACFA0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Odorant responses of olfactory sensory neurons expressing the odorant receptor MOR23: a patch clamp analysis in gene-targeted mice.
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Publication state
Published
Issued date
07/02/2006
Peer-reviewed
Oui
Volume
103
Number
6
Pages
1970-1975
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
A glomerulus in the mammalian olfactory bulb receives axonal inputs from olfactory sensory neurons (OSNs) that express the same odorant receptor (OR). Glomeruli are generally thought to represent functional units of olfactory coding, but there are no data on the electrophysiological properties of OSNs that express the same endogenous OR. Here, using patch clamp recordings in an intact epithelial preparation, we directly measured the transduction currents and receptor potentials from the dendritic knobs of mouse OSNs that express the odorant receptor MOR23 along with the green fluorescent protein. All of the 53 cells examined responded to lyral, a known ligand for MOR23. There were profound differences in response kinetics, particularly in the deactivation phase. The cells were very sensitive to lyral, with some cells responding to as little as 10 nM. The dynamic range was unexpectedly broad, with threshold and saturation in individual cells often covering three log units of lyral concentration. The potential causes and biological significance of this cellular heterogeneity are discussed. Patch clamp recording from OSNs that express a defined OR provides a powerful approach to investigate the sensory inputs to individual glomeruli.
Keywords
Aldehydes/pharmacology, Animals, Cyclohexenes, Electrophysiology, Kinetics, Mice, Neurons, Afferent/metabolism, Olfactory Bulb/cytology, Olfactory Bulb/drug effects, Olfactory Bulb/metabolism, Patch-Clamp Techniques, Receptors, Odorant/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/10/2018 8:22
Last modification date
25/05/2024 6:14