Vasopressin-inducible ubiquitin-specific protease 10 increases ENaC cell surface expression by deubiquitylating and stabilizing sorting nexin 3

Details

Serval ID
serval:BIB_7EF78ADFF96E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Vasopressin-inducible ubiquitin-specific protease 10 increases ENaC cell surface expression by deubiquitylating and stabilizing sorting nexin 3
Journal
American Journal of Physiology - Renal Physiology
Author(s)
Boulkroun S., Ruffieux-Daidie D., Vitagliano J. J., Poirot O., Charles R. P., Lagnaz D., Firsov D., Kellenberger S., Staub O.
ISSN
0363-6127
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
295
Number
4
Pages
F889-F900
Language
english
Abstract
Adjustment of Na+ balance in extracellular fluids is achieved by regulated Na+ transport involving the amiloride-sensitive epithelial Na+ channel (ENaC) in the distal nephron. In this context, ENaC is controlled by a number of hormones, including vasopressin, which promotes rapid translocation of water and Na+ channels to the plasma membrane and long-term effects on transcription of vasopressin-induced and -reduced transcripts. We have identified a mRNA encoding the deubiquitylating enzyme ubiquitin-specific protease 10 (Usp10), whose expression is increased by vasopressin at both the mRNA and the protein level. Coexpression of Usp10 in ENaC-transfected HEK-293 cells causes a more than fivefold increase in amiloride-sensitive Na+ currents, as measured by whole cell patch clamping. This is accompanied by a three- to fourfold increase in surface expression of alpha- and gamma-ENaC, as shown by cell surface biotinylation experiments. Although ENaC is well known to be regulated by its direct ubiquitylation, Usp10 does not affect the ubiquitylation level of ENaC, suggesting an indirect effect. A two-hybrid screen identified sorting nexin 3 (SNX3) as a novel substrate of Usp10. We show that it is a ubiquitylated protein that is degraded by the proteasome; interaction with Usp10 leads to its deubiquitylation and stabilization. When coexpressed with ENaC, SNX3 increases the channel's cell surface expression, similarly to Usp10. In mCCD(cl1) cells, vasopressin increases SNX3 protein but not mRNA, supporting the idea that the vasopressin-induced Usp10 deubiquitylates and stabilizes endogenous SNX3 and consequently promotes cell surface expression of ENaC
Keywords
Animals , Antidiuretic Agents , Carrier Proteins , Cell Line , Cell Membrane , cytology , drug effects , Epithelial Sodium Channel , genetics , Humans , Kidney , Membrane Potentials , metabolism , Mice , pharmacology , physiology , Rats , RNA,Messenger , Sodium , Switzerland , Transfection , Two-Hybrid System Techniques , Ubiquitin , Ubiquitin Thiolesterase , Vasopressins , Vesicular Transport Proteins
Pubmed
Web of science
Create date
29/01/2009 22:13
Last modification date
20/08/2019 14:39
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