Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized <sup>13</sup>C magnetic resonance.

Details

Ressource 1Download: 28916775_BIB_7E6E52E2D4F7.pdf (1590.90 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_7E6E52E2D4F7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized <sup>13</sup>C magnetic resonance.
Journal
Scientific reports
Author(s)
Mishkovsky M., Anderson B., Karlsson M., Lerche M.H., Sherry A.D., Gruetter R., Kovacs Z., Comment A.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
15/09/2017
Peer-reviewed
Oui
Volume
7
Number
1
Pages
11719
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The mammalian brain relies primarily on glucose as a fuel to meet its high metabolic demand. Among the various techniques used to study cerebral metabolism, <sup>13</sup> C magnetic resonance spectroscopy (MRS) allows following the fate of <sup>13</sup> C-enriched substrates through metabolic pathways. We herein demonstrate that it is possible to measure cerebral glucose metabolism in vivo with sub-second time resolution using hyperpolarized <sup>13</sup> C MRS. In particular, the dynamic <sup>13</sup> C-labeling of pyruvate and lactate formed from <sup>13</sup> C-glucose was observed in real time. An ad-hoc synthesis to produce [2,3,4,6,6- <sup>2</sup> H <sub>5</sub> , 3,4- <sup>13</sup> C <sub>2</sub> ]-D-glucose was developed to improve the <sup>13</sup> C signal-to-noise ratio as compared to experiments performed following [U- <sup>2</sup> H <sub>7</sub> , U- <sup>13</sup> C]-D-glucose injections. The main advantage of only labeling C3 and C4 positions is the absence of <sup>13</sup> C- <sup>13</sup> C coupling in all downstream metabolic products after glucose is split into 3-carbon intermediates by aldolase. This unique method allows direct detection of glycolysis in vivo in the healthy brain in a noninvasive manner.
Keywords
Animals, Brain/metabolism, Carbon-13 Magnetic Resonance Spectroscopy/methods, Glucose/metabolism, Glycolysis, Lactic Acid/metabolism, Mice, Pyruvic Acid/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
05/10/2017 10:33
Last modification date
20/08/2019 15:39
Usage data