Investigating Antifungal Susceptibility in Candida Species With MALDI-TOF MS-Based Assays.

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Version: Final published version
License: Not specified
Serval ID
serval:BIB_7E4799E9694B
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Investigating Antifungal Susceptibility in Candida Species With MALDI-TOF MS-Based Assays.
Journal
Frontiers in cellular and infection microbiology
Author(s)
Delavy M., Dos Santos A.R., Heiman C.M., Coste A.T.
ISSN
2235-2988 (Electronic)
ISSN-L
2235-2988
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
9
Pages
19
Language
english
Notes
Publication types: Journal Article ; Review
Publication Status: epublish
Abstract
Half of invasive fungal infections lead to death. Amongst pathogenic fungi, the most widespread species belong to the Candida genus and vary in their susceptibility to antifungal drugs. The emergence of antifungal resistance has become a major clinical problem. Therefore, the definition of susceptibility patterns is crucial for the survival of patients and the monitoring of resistance epidemiology. Although, most routinely used methods of AntiFungal Susceptibility Testing (AFST) have reached their limits, the rediscovery of Matrix Associated Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) in the field of mycology provides a promising alternative for the study of antifungal resistance. MALDI-TOF MS is already used in mycology for fungal identification, which permits to highlight inherent antifungal resistance. However, the main concern of clinicians is the rise of acquired antifungal resistance and the time needed for their detection. For this purpose, MALDI-TOF MS has been shown to be an accurate tool for AFST, presenting numerous advantages in comparison to commonly used techniques. Finally, MALDI-TOF MS could be used directly to detect resistance mechanisms through typing. Consequently, MALDI-TOF MS offers new perspectives in the context of healthcare associated outbreaks of emerging multi-drug resistant fungi, such as C. auris. As a proof of concept, we will illustrate the current and future benefits in using and adapting MALDI-TOF MS-based assays to define the susceptibility pattern of C. auris, by species identification, AFST, and typing.
Keywords
Antifungal Agents/pharmacology, Candida/classification, Candida/drug effects, Candida/isolation & purification, Candidiasis, Invasive/microbiology, Humans, Microbial Sensitivity Tests/methods, Mycological Typing Techniques/methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods, Candida spp., MALDI-TOF MS, antifungal resistance, antifungal susceptibility testing, identification, typing
Pubmed
Web of science
Open Access
Yes
Create date
18/02/2019 16:28
Last modification date
16/12/2019 6:19
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