Allergen-specific antibody and cytokine responses, mast cell reactivity and intestinal permeability upon oral challenge of sensitized and tolerized mice.

Details

Serval ID
serval:BIB_7E46A71317DA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Allergen-specific antibody and cytokine responses, mast cell reactivity and intestinal permeability upon oral challenge of sensitized and tolerized mice.
Journal
Clinical and Experimental Allergy : Journal of the British Society For Allergy and Clinical Immunology
Author(s)
Perrier C., Thierry A.C., Mercenier A., Corthésy B.
ISSN
1365-2222[electronic], 0954-7894[linking]
Publication state
Published
Issued date
2010
Volume
40
Number
1
Pages
153-162
Language
english
Notes
BIB_5977E173969F
Abstract
BACKGROUND: Food allergy has reached an epidemic level in westernized countries and although central mechanisms have been described, the variability associated with genetic diversity underscores the still unresolved complexity of these disorders. OBJECTIVE: To develop models of food allergy and oral tolerance, both strictly induced by the intestinal route, and to compare antigen-specific responses. METHODS: BALB/c mice were mucosally sensitized to ovalbumin (OVA) in the presence of the mucosal adjuvant cholera toxin, or tolerized by intra-gastric administrations of OVA alone. Antibody titres and cytokines were determined by ELISA, and allergic status was determined through several physiologic parameters including decline in temperature, diarrhoea, mast cell degranulation and intestinal permeability. RESULTS: OVA-specific antibodies (IgE, IgGs and IgA in serum and feces) were produced in sensitized mice exclusively. Upon intra-gastric challenge with OVA, sensitized mice developed anaphylactic reactions associated with a decline of temperature, diarrhoea, degranulation of mast cells, which were only moderately recruited in the small intestine, and increased intestinal permeability. Cytokines produced by immune cells from sensitized mice included T-helper type 2 cytokines (IL-5, IL-13), but also IL-10, IFN-gamma and IL-17. In contrast, all markers of allergy were totally absent in tolerized animals, and yet the latter were protected from subsequent sensitization, demonstrating that oral tolerance took place efficiently. CONCLUSION: This work allows for the first time an appropriate comparison between sensitized and tolerized BALB/c mice towards OVA. It highlights important differences from other models of allergy, and thus questions some of the generally accepted notions of allergic reactions, such as the protective role of IFN-gamma, the importance of antigen-specific secretory IgA and the role of mucosal mast cells in intestinal anaphylaxis. In addition, it suggests that IL-17 might be an effector cytokine in food allergy. Finally, it demonstrates that intestinal permeability towards the allergen is increased during challenge.
Keywords
Administration, Oral, Allergens/immunology, Animals, Antibodies/blood, Antibodies/immunology, Antibody Specificity, Cell Degranulation, Cells, Cultured, Cytokines/immunology, Disease Models, Animal, Female, Food Hypersensitivity/immunology, Immune Tolerance, Immunoglobulin A, Secretory/immunology, Interleukin-17/biosynthesis, Interleukin-17/immunology, Intestinal Mucosa/immunology, Intestinal Mucosa/pathology, Lymphocytes/immunology, Lymphocytes/metabolism, Mast Cells/immunology, Mice, Mice, Inbred BALB C, Ovalbumin/immunology
Pubmed
Web of science
Open Access
Yes
Create date
15/06/2011 10:27
Last modification date
20/08/2019 14:39
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