Microtubule Associated Protein 1b (MAP1B) Is a Marker of the Microtubular Cytoskeleton in Podocytes but Is Not Essential for the Function of the Kidney Filtration Barrier in Mice.

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Serval ID
serval:BIB_7E34816FADC3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Microtubule Associated Protein 1b (MAP1B) Is a Marker of the Microtubular Cytoskeleton in Podocytes but Is Not Essential for the Function of the Kidney Filtration Barrier in Mice.
Journal
Plos One
Author(s)
Gödel M., Temerinac D., Grahammer F., Hartleben B., Kretz O., Riederer B.M., Propst F., Kohl S., Huber T.B.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
10
Number
10
Pages
e0140116
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: epublish
Abstract
Podocytes are essential for the function of the kidney glomerular filter. A highly differentiated cytoskeleton is requisite for their integrity. Although much knowledge has been gained on the organization of cortical actin networks in podocyte's foot processes, less is known about the molecular organization of the microtubular cytoskeleton in primary processes and the cell body. To gain an insight into the organization of the microtubular cytoskeleton of the podocyte, we systematically analyzed the expression of microtubule associated proteins (Maps), a family of microtubules interacting proteins with known functions as regulator, scaffold and guidance proteins. We identified microtubule associated protein 1b (MAP1B) to be specifically enriched in podocytes in human and rodent kidney. Using immunogold labeling in electron microscopy, we were able to demonstrate an enrichment of MAP1B in primary processes. A similar association of MAP1B with the microtubule cytoskeleton was detected in cultured podocytes. Subcellular distribution of MAP1B HC and LC1 was analyzed using a double fluorescent reporter MAP1B fusion protein. Subsequently we analyzed mice constitutively depleted of MAP1B. Interestingly, MAP1B KO was not associated with any functional or structural alterations pointing towards a redundancy of MAP proteins in podocytes. In summary, we established MAP1B as a specific marker protein of the podocyte microtubular cytoskeleton.
Keywords
Animals, Biomarkers, Cells, Cultured, Female, Glomerular Filtration Barrier/metabolism, Glomerular Filtration Rate, Male, Mice, Inbred C57BL, Mice, Knockout, Microtubule-Associated Proteins, Microtubules/metabolism, Microtubules/ultrastructure, Organ Specificity, Podocytes/metabolism, Podocytes/ultrastructure
Pubmed
Web of science
Open Access
Yes
Create date
03/11/2015 17:30
Last modification date
20/08/2019 14:39
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