Association of nuclear matrix proteins with granular and threaded nuclear bodies in cell lines undergoing apoptosis.

Details

Serval ID
serval:BIB_7DA4462ABAB0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Association of nuclear matrix proteins with granular and threaded nuclear bodies in cell lines undergoing apoptosis.
Journal
Experimental Cell Research
Author(s)
Zweyer M., Riederer B.M., Ochs R.L., Fackelmayer F.O., Kohwi-Shigematsu T., Bareggi R., Narducci P., Martelli A.M.
ISSN
0014-4827 (Print)
ISSN-L
0014-4827
Publication state
Published
Issued date
1997
Volume
230
Number
2
Pages
325-336
Language
english
Abstract
The granules which appear in the nucleolar area in apoptotic HL-60 cells after camptothecin administration (Zweyer et al., Exp. Cell Res. 221,27-40, 1995) were detected also in several other cell lines induced to undergo apoptosis by different stimuli, such as MOLT-4 treated with staurosporine, K-562 incubated with actinomycin D, P-815 exposed to temperature causing heat shock, Jurkat cells treated with EGTA, U-937 growing in the presence of cycloheximide and tumor necrosis factor-alpha, and HeLa cells treated with etoposide. Using immunoelectron microscopy techniques, we demonstrate that, besides the already described nuclear matrix proteins p125 and p160, these granules contain other nucleoskeletal polypeptides such as proliferating cell nuclear antigen, a component of ribonucleoprotein particles, a 105-kDa constituent of nuclear spliceosomes, and the 240-kDa nuclear mitotic apparatus-associated protein referred to as NuMA. Moreover, we also found in the granules SAF-A/hn-RNP-U and SATB1 proteins, two polypeptides that have been reported to bind scaffold-associated regions DNA sequences in vitro, thus mediating the formation of looped DNA structures in vivo. Fibrillarin and coilin are not present in these granules or the PML protein. Thus, the granules seen during the apoptotic process apparently are different from coiled bodies or other types of nuclear bodies. Furthermore, these granules do not contain chromatin components such as histones and DNA. Last, Western blotting analysis revealed that nuclear matrix proteins present in the granules are not proteolytically degraded except for the NuMA polypeptide. We propose that these granules might represent aggregates of nuclear matrix proteins forming during the apoptotic process. Moreover, since the granules are present in several cell lines undergoing apoptosis, they could be considered a previously unrecognized morphological hallmark of the apoptotic process.
Keywords
Antigens, Nuclear, Apoptosis/physiology, Biological Markers/analysis, Cell Line, Chromatin/metabolism, Chromosomal Proteins, Non-Histone/analysis, DNA, Endopeptidases/metabolism, HL-60 Cells, Histones/metabolism, Humans, Inclusion Bodies/metabolism, Jurkat Cells, Neoplasm Proteins, Nuclear Proteins/analysis, Transcription Factors/analysis, Tumor Cells, Cultured, Tumor Suppressor Proteins
Pubmed
Web of science
Create date
24/01/2008 14:34
Last modification date
20/08/2019 14:38
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