Integration analysis of pSK41 in the chromosome of a methicillin-resistant Staphylococcus aureus K-1.

Details

Serval ID
serval:BIB_7D9488E53BD1
Type
Article: article from journal or magazin.
Collection
Publications
Title
Integration analysis of pSK41 in the chromosome of a methicillin-resistant Staphylococcus aureus K-1.
Journal
Journal of basic microbiology
Author(s)
McElgunn C.J., Zahurul M., Bhuyian A., Sugiyama M.
ISSN
0233-111X (Print)
ISSN-L
0233-111X
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
42
Number
3
Pages
190-200
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
An integrate of pUB110 carrying aadD and blmS genes conferring resistance to 4'-OH containing aminoglycosides and bleomycin, respectively, is found in the Staphylococcal conjugative plasmid pSK41 and in the mec chromosomal region. In this study, we found an integrated copy of pSK41 in the chromosome of an arbekacin-resistant MRSA strain, K-1, clinically isolated in Japan. This is the first report of the chromosomal integration of this large plasmid in MRSA. Analysis of the nucleotide sequence and restriction map revealed a complete homology of the blmS-containing region of the integrate to wild type pSK41, except for the deletion of aadD and a part of the repU gene. In addition, we have discovered an insertion of IS1182 within the pSK41 integrate. Pulse-field gel electrophoresis-Southern analysis showed that the integration occurred outside the mec region. We also found that deletion of aadD resulted in an aberration of blmS transcription.

Keywords
Acetyltransferases/genetics, Aminoglycosides, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Base Sequence, Bleomycin/pharmacology, Chromosomes, Bacterial/genetics, Conjugation, Genetic, DNA Transposable Elements, DNA, Bacterial, Dibekacin/analogs & derivatives, Dibekacin/pharmacology, Drug Resistance, Multiple, Bacterial/genetics, Gene Deletion, Gene Expression Regulation, Bacterial, Humans, Methicillin Resistance, Molecular Sequence Data, Plasmids/genetics, Restriction Mapping, Sequence Analysis, DNA, Staphylococcus aureus/drug effects, Staphylococcus aureus/genetics, Transcription, Genetic
Pubmed
Web of science
Create date
01/03/2018 16:44
Last modification date
20/08/2019 15:38
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