CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL

Details

Serval ID
serval:BIB_7D6EDC319849
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL
Journal
Cellular Immunology
Author(s)
Wang  J. K., Zhu  B., Ju  S. T., Tschopp  J., Marshak-Rothstein  A.
ISSN
0008-8749 (Print)
Publication state
Published
Issued date
08/1997
Volume
179
Number
2
Pages
153-64
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Aug 1
Abstract
Naive CD4(+) T cells proliferate strongly in response to superantigens such as staphylococcal enterotoxin B (SEB). When these cells are rested and challenged a second time, they undergo activation-induced cell death (AICD). Fas/FasL interactions have been shown to mediate AICD, even though the level of Fas expression in the 2 degrees SEB responder populations is no higher than in the 1 degrees cultures. To determine whether the dissimilarity between the 1 degrees and 2 degrees cultures could be attributed to differences in FasL cytotoxic activity or in the sensitivity of the Fas apoptosis signaling pathway, we compared these parameters during the 1 degrees and 2 degrees responses of lpr and gld CD4+ T cells (which do not undergo AICD due to a lack of Fas and an inactive FasL, respectively) so that each parameter could be evaluated independently. The results demonstrate that 2 degrees responders both express a higher level of functional FasL and are more sensitive to FasL-mediated killing. These findings account for the differences between the 1 degrees and 2 degrees responses of CD4+ T cells to superantigen. In addition, we found that the apparent level of FasL-mediated cytotoxic activity in the 2 degrees lpr CD4+ T cell population is much higher than that of wild-type cells, suggesting that deficient Fas expression leads to inordinately high levels of FasL expression or subsaturation of FasL binding sites.
Keywords
Animals Antigens, CD95/*biosynthesis/genetics/*toxicity Antigens, Surface/biosynthesis/genetics/physiology Apoptosis/immunology CD4-Positive T-Lymphocytes/*immunology/metabolism *Cytotoxicity, Immunologic Enterotoxins/immunology/pharmacology Fas Ligand Protein Kinetics Ligands *Lymphocyte Activation Membrane Glycoproteins/*biosynthesis/genetics/*toxicity Mice Mice, Inbred MRL lpr Protein Biosynthesis RNA/biosynthesis Staphylococcus aureus/immunology Superantigens/immunology/*pharmacology Time Factors
Pubmed
Web of science
Create date
24/01/2008 15:18
Last modification date
20/08/2019 14:38
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