The discriminatory power of MALDI-TOF mass spectrometry to differentiate between isogenic teicoplanin-susceptible and teicoplanin-resistant strains of methicillin-resistant Staphylococcus aureus.

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Serval ID
serval:BIB_7D4D66D49213
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The discriminatory power of MALDI-TOF mass spectrometry to differentiate between isogenic teicoplanin-susceptible and teicoplanin-resistant strains of methicillin-resistant Staphylococcus aureus.
Journal
FEMS Microbiology Letters
Author(s)
Majcherczyk P.A., McKenna T., Moreillon P., Vaudaux P.
ISSN
0378-1097[print], 0378-1097[linking]
Publication state
Published
Issued date
2006
Volume
255
Number
2
Pages
233-239
Language
english
Abstract
To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More important changes in MALDI-TOF MS spectra were found with strains differing in methicillin than in teicoplanin resistance. In comparison, very minor or no changes were recorded in pulsed-field gel electrophoresis profiles or peptidoglycan muropeptide digest patterns of these strains, respectively. MALDI-TOF MS might be useful to detect subtle strain-specific differences in ionizable components released from bacterial surfaces and not from their peptidoglycan network.
Keywords
Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques/methods, Drug Resistance, Bacterial, Electrophoresis, Gel, Pulsed-Field, Humans, Methicillin Resistance, Muramidase/metabolism, Peptides/analysis, Peptidoglycan/chemistry, Peptidoglycan/metabolism, Sensitivity and Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods, Staphylococcus aureus/chemistry, Staphylococcus aureus/classification, Teicoplanin/pharmacology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 13:54
Last modification date
25/09/2019 6:09
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