The major surface protein of Leishmania promastigotes is a fibronectin-like molecule

Details

Serval ID
serval:BIB_7CF252A68DA6
Type
Article: article from journal or magazin.
Collection
Publications
Title
The major surface protein of Leishmania promastigotes is a fibronectin-like molecule
Journal
European Journal of Immunology
Author(s)
Rizvi  F. S., Ouaissi  M. A., Marty  B., Santoro  F., Capron  A.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
03/1988
Volume
18
Number
3
Pages
473-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
The major surface glycoprotein of Leishmania chagasi promastigotes showed crossreactivity with fibronectin (Fn), a large glycoprotein that is a major constituent of the extracellular matrix of most mononuclear cells. Polyclonal and monoclonal antibodies against Fn precipitated two molecules of 63-58 kDa from the lysates of both 125I and [35S]methionine-labeled promastigotes. In addition, a monoclonal antibody against a 15-kDa fragment of Fn containing the Arg-Gly-Asp-Ser (RGDS) sequence and several polyclonal monospecific mouse antibodies against a synthetic RGDS peptide also recognized the above two molecules. The attachment of Leishmania promastigotes to mouse peritoneal macrophages in vitro was partially inhibited when promastigotes were treated with F(ab')2 fragment of an anti-Fn IgG. Identical results were obtained by saturating the Fn receptors on macrophages using different peptides containing the RGDS sequence. Moreover, antigen preparations rich in glycoprotein 63 could efficiently promote the attachment and spreading of 3T3 mouse fibroblasts to surfaces coated with the antigen. These results clearly suggest that the gp63 of L. chagasi promastigotes is an Fn-like molecule that shares certain biological and molecular characteristics with Fn.
Keywords
Animals Antibodies, Protozoan/immunology Antigens, Protozoan/*immunology Antigens, Surface/*immunology Cell Line Cross Reactions Fibroblasts Fibronectins/*immunology Glycoproteins/*immunology Immunoglobulin Fab Fragments/immunology Leishmania/growth & development/*immunology Mice Oligopeptides/metabolism Receptors, Fibronectin Receptors, Immunologic/metabolism
Pubmed
Web of science
Create date
28/01/2008 10:31
Last modification date
03/03/2018 18:38
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