An agarose matrix facilitates sectioning of tissue microarray blocks.

Details

Serval ID
serval:BIB_7CEEDFCE46F6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An agarose matrix facilitates sectioning of tissue microarray blocks.
Journal
Journal of Histochemistry and Cytochemistry
Author(s)
Yan P., Seelentag W., Bachmann A., Bosman F.T.
ISSN
0022-1554
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
55
Number
1
Pages
21-24
Language
english
Abstract
Tissue microarray (TMA) is a powerful, high-throughput technique for in situ investigation of biomarkers in many tissue samples in a paraffin block by immunohistochemistry or fluorescence in situ hybridization (FISH), and has rapidly become the standard in marker studies. One of the difficult steps in the procedure is the sectioning of array blocks and mounting of sections using special slides and/or adhesive-coated tape, which demands specific experience and is time-consuming. We report an arraying method that allows melting of the receiving paraffin block and subsequent sectioning like any ordinary paraffin-embedded tissue block. The major difference from the standard microarray technique is the use of an agarose matrix in the recipient block. The agarose matrix allows melting of the paraffin without disturbing the array, resulting in perfect integration of the tissue cores. The agarose-paraffin TMA blocks limit tissue core loss during cutting, mounting, or immunohistochemical or FISH staining and better maintains the array.
Keywords
Hepatocytes, Immunohistochemistry, In Situ Hybridization, Fluorescence, Keratin-6, Keratin-7, Microtomy, Paraffin, Receptor, Epidermal Growth Factor, Sepharose, Serum Amyloid A Protein, Tissue Array Analysis, Tissue Embedding
Pubmed
Web of science
Create date
29/01/2008 18:33
Last modification date
20/08/2019 14:38
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