Expression of the proto-oncogene c-myc in human stenotic aortocoronary bypass grafts.

Details

Serval ID
serval:BIB_7BBBE3AE33A3
Type
Article: article from journal or magazin.
Collection
Publications
Title
Expression of the proto-oncogene c-myc in human stenotic aortocoronary bypass grafts.
Journal
Pathology, Research and Practice
Author(s)
Hilker M., Tellmann G., Buerke M., Moersig W., Oelert H., Lehr H.A., Hake U.
ISSN
0344-0338 (Print)
ISSN-L
0344-0338
Publication state
Published
Issued date
2001
Volume
197
Number
12
Pages
811-816
Language
english
Abstract
Proliferation and differentiation of vascular smooth muscle cells (VSMC) are central events in vascular pathobiology and play a major role in the development of stenotic and restenotic lesions. The proto-oncogene c-myc and other early cell cycle-regulating genes have been implicated in the induction of cell proliferation and differentiation under diverse pathophysiological conditions. In the present study we analyzed c-myc mRNA expression by indirect nonradioactive in situ hybridization technique (NISH) in human stenotic venous bypass grafts (n = 32) retrieved during re-do operations of coronary artery disease and compared the results with 28 native veins (vena saphena magna) from the same patients. Stenotic bypass grafts showed enhanced c-myc expression located predominantly in VSMC in the media and neointima (severity score: ++-+++, 32/32 stenotic veins). In native veins we observed only low levels of c-myc mRNA (severity score: +, 28/28 native veins), all signals were restricted to endothelial cells of either the innermost intimal layer or of the vasa vasorum. Our in situ hybridization studies demonstrate enhanced mRNA expression of the proto-oncogene c-myc in stenotic venous bypass grafts. These results suggest that--in analogy to other pathophysiological conditions--c-myc exerts essential regulatory functions in cellular events operative during the initiation and progression of venous bypass graft disease.
Keywords
Adult, Aged, Blood Vessel Prosthesis, Coronary Artery Bypass, Female, Graft Occlusion, Vascular/metabolism, Graft Occlusion, Vascular/pathology, Humans, In Situ Hybridization, Male, Middle Aged, Proto-Oncogene Proteins c-myc/genetics, Proto-Oncogene Proteins c-myc/metabolism, RNA, Messenger/metabolism, Saphenous Vein/metabolism, Saphenous Vein/pathology
Pubmed
Create date
26/11/2011 13:46
Last modification date
20/08/2019 14:37
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