Macrophages promote epithelial repair through hepatocyte growth factor secretion.

Details

Serval ID
serval:BIB_7B92562F4EAF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Macrophages promote epithelial repair through hepatocyte growth factor secretion.
Journal
Clinical and Experimental Immunology
Author(s)
D'Angelo F., Bernasconi E., Schäfer M. , Moyat M., Michetti P., Maillard M.H., Velin D.
ISSN
1365-2249 (Electronic)
ISSN-L
0009-9104
Publication state
Published
Issued date
2013
Volume
174
Number
1
Pages
60-72
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish. PDF type: Original article
Abstract
Macrophages play a critical role in intestinal wound repair. However, the mechanisms of macrophage-assisted wound repair remain poorly understood. We aimed to characterize more clearly the repair activities of murine and human macrophages. Murine macrophages were differentiated from bone marrow cells and human macrophages from monocytes isolated from peripheral blood mononuclear cells of healthy donors (HD) or Crohn's disease (CD) patients or isolated from the intestinal mucosa of HD. In-vitro models were used to study the repair activities of macrophages. We found that murine and human macrophages were both able to promote epithelial repair in vitro. This function was mainly cell contact-independent and relied upon the production of soluble factors such as the hepatocyte growth factor (HGF). Indeed, HGF-silenced macrophages were less capable of promoting epithelial repair than control macrophages. Remarkably, macrophages from CD patients produced less HGF than their HD counterparts (HGF level: 84âeuro0/00±âeuro0/0027âeuro0/00pg/mg of protein and 45âeuro0/00±âeuro0/0034âeuro0/00pg/mg of protein, respectively, for HD and CD macrophages, Pâeuro0/00<âeuro0/000·009) and were deficient in promoting epithelial repair (repairing activity: 90·1âeuro0/00±âeuro0/004·6 and 75·8âeuro0/00±âeuro0/008·3, respectively, for HD and CD macrophages, Pâeuro0/00<âeuro0/000·0005). In conclusion, we provide evidence that macrophages act on wounded epithelial cells to promote epithelial repair through the secretion of HGF. The deficiency of CD macrophages to secrete HGF and to promote epithelial repair might contribute to the impaired intestinal mucosal healing in CD patients.
Pubmed
Web of science
Open Access
Yes
Create date
03/10/2013 17:58
Last modification date
20/08/2019 15:37
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