Analysis of free and esterified sterols in fats and oils by flash chromatography, gas chromatography and electrospray tandem mass spectrometry.
Details
Serval ID
serval:BIB_7B7165AAC2D9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of free and esterified sterols in fats and oils by flash chromatography, gas chromatography and electrospray tandem mass spectrometry.
Journal
Journal of chromatography. A
ISSN
0021-9673 (Print)
ISSN-L
0021-9673
Publication state
Published
Issued date
23/11/2001
Peer-reviewed
Oui
Volume
935
Number
1-2
Pages
237-248
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
A method for the analysis of free and esterified sterols has been developed. Fat or oil samples were separated on solid-phase extraction silica gel columns into a sterol ester fraction, a fraction of triacylglycerols, and a free sterol fraction containing partial acylglycerols and residual triacylglycerols. Sterol esters and acylglycerols of the free sterol fraction were transesterified to methyl esters. The fatty acid methyl esters from sterol ester fraction and the free sterols from sterol ester fraction and free sterol fraction were determined by GLC. Precursor ion electrospray MS-MS of sterol fragment ions of sterol ester fractions were recorded and used for determination of sterol ester proportions in butterfat and vegetable oil samples.
Keywords
Chromatography/methods, Chromatography, Gas/methods, Esterification, Fats/chemistry, Oils/chemistry, Spectrometry, Mass, Electrospray Ionization/methods, Sterols/analysis
Pubmed
Web of science
Create date
02/05/2017 14:29
Last modification date
20/08/2019 15:37
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