Label-free 3D molecular imaging of living tissues using Raman spectral projection tomography.

Details

Serval ID
serval:BIB_7AD5A1901039
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Label-free 3D molecular imaging of living tissues using Raman spectral projection tomography.
Journal
Nature communications
Author(s)
Stepula E., Walther A.R., Jensen M., Mehrotra D.R., Yuan M.H., Pedersen S.V., Kumar V., Gentleman E., Albro M.B., Hedegaard MAB, Bergholt M.S.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
09/09/2024
Peer-reviewed
Oui
Volume
15
Number
1
Pages
7717
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The ability to image tissues in three dimensions (3D) with label-free molecular contrast at the mesoscale would be a valuable capability in biology and biomedicine. Here, we introduce Raman spectral projection tomography (RSPT) for volumetric molecular imaging with optical sub-millimeter spatial resolution. We have developed a RSPT imaging instrument capable of providing 3D molecular contrast in transparent and semi-transparent samples. We also created a computational pipeline for multivariate reconstruction to extract label-free spatial molecular information from Raman projection data. Using these tools, we demonstrate imaging and visualization of phantoms of various complex shapes with label-free molecular contrast. Finally, we apply RSPT as a tool for imaging of molecular gradients and extracellular matrix heterogeneities in fixed and living tissue-engineered constructs and explanted native cartilage tissues. We show that there exists a favorable balance wherein employing Raman spectroscopy, with its advantages in live cell imaging and label-free molecular contrast, outweighs the reduction in imaging resolution and blurring caused by diffuse photon propagation. Thus, RSPT imaging opens new possibilities for label-free molecular monitoring of tissues.
Keywords
Spectrum Analysis, Raman/methods, Imaging, Three-Dimensional/methods, Animals, Molecular Imaging/methods, Phantoms, Imaging, Tissue Engineering/methods, Humans, Tomography/methods, Cartilage/diagnostic imaging, Cartilage/metabolism, Extracellular Matrix/metabolism, Mice
Pubmed
Open Access
Yes
Create date
09/09/2024 13:51
Last modification date
05/10/2024 7:02
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