Label-free 3D molecular imaging of living tissues using Raman spectral projection tomography.
Details
Serval ID
serval:BIB_7AD5A1901039
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Label-free 3D molecular imaging of living tissues using Raman spectral projection tomography.
Journal
Nature communications
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
09/09/2024
Peer-reviewed
Oui
Volume
15
Number
1
Pages
7717
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
The ability to image tissues in three dimensions (3D) with label-free molecular contrast at the mesoscale would be a valuable capability in biology and biomedicine. Here, we introduce Raman spectral projection tomography (RSPT) for volumetric molecular imaging with optical sub-millimeter spatial resolution. We have developed a RSPT imaging instrument capable of providing 3D molecular contrast in transparent and semi-transparent samples. We also created a computational pipeline for multivariate reconstruction to extract label-free spatial molecular information from Raman projection data. Using these tools, we demonstrate imaging and visualization of phantoms of various complex shapes with label-free molecular contrast. Finally, we apply RSPT as a tool for imaging of molecular gradients and extracellular matrix heterogeneities in fixed and living tissue-engineered constructs and explanted native cartilage tissues. We show that there exists a favorable balance wherein employing Raman spectroscopy, with its advantages in live cell imaging and label-free molecular contrast, outweighs the reduction in imaging resolution and blurring caused by diffuse photon propagation. Thus, RSPT imaging opens new possibilities for label-free molecular monitoring of tissues.
Keywords
Spectrum Analysis, Raman/methods, Imaging, Three-Dimensional/methods, Animals, Molecular Imaging/methods, Phantoms, Imaging, Tissue Engineering/methods, Humans, Tomography/methods, Cartilage/diagnostic imaging, Cartilage/metabolism, Extracellular Matrix/metabolism, Mice
Pubmed
Open Access
Yes
Create date
09/09/2024 13:51
Last modification date
05/10/2024 7:02