Antigenic crossreactivity of the alpha subunit of complement component C8 with the cysteine-rich domain shared by complement component C9 and low density lipoprotein receptor

Details

Serval ID
serval:BIB_7AC909A1305B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Antigenic crossreactivity of the alpha subunit of complement component C8 with the cysteine-rich domain shared by complement component C9 and low density lipoprotein receptor
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Tschopp  J., Mollnes  T. E.
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
06/1986
Volume
83
Number
12
Pages
4223-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
Complement component C9 contains two distinct cysteine-rich domains exhibiting high sequence resemblance to a domain present in the low density lipoprotein (LDL) receptor and epidermal growth factor precursor, respectively. Antibodies were raised against a peptide corresponding to the most conserved region of the LDL receptor/C9-homology segment. The antibodies were shown by immunoblotting to bind specifically to C9 but also to crossreact with C8 alpha, the alpha subunit of complement component C8. Moreover, a monoclonal antibody to a neoantigen present in polymerized C9 bound to an epitope exposed on C8 within the C5b-8 complex but buried in monomeric C8, suggesting that C8 and C9 undergo similar conformational changes during membrane-attack-complex assembly. Isolated C8 alpha-gamma exhibited the propensity to polymerize in the presence of Zn2+ and urea, as already demonstrated for C9. These data indicate that C8 alpha is closely related, both structurally and functionally, to C9.
Keywords
Complement C8/*immunology Complement C9/*immunology Complement Membrane Attack Complex Complement System Proteins/immunology Cross Reactions Cysteine Epitopes Humans Macromolecular Substances Oligopeptides/chemical synthesis/immunology Receptors, LDL/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:18
Last modification date
20/08/2019 14:36
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