Secretory IgA mediates bacterial translocation to dendritic cells in mouse Peyer's patches with restriction to mucosal compartment

Details

Serval ID
serval:BIB_7A9815A4CCAF
Type
Article: article from journal or magazin.
Collection
Publications
Title
Secretory IgA mediates bacterial translocation to dendritic cells in mouse Peyer's patches with restriction to mucosal compartment
Journal
Journal of Immunology
Author(s)
Kadaoui  K. A., Corthesy  B.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
2007
Volume
179
Number
11
Pages
7751-7757
Notes
DA - 20071120
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Antibodies)
RN - 0 (Immunoglobulin A, Secretory)
SB - AIM
SB - IM
Abstract
In addition to fulfilling its function of immune exclusion at mucosal surfaces, secretory IgA (SIgA) Ab exhibits the striking feature to adhere selectively to M cells in the mouse and human intestinal Peyer's patches (PPs). Subsequent uptake drives the SIgA Ab to dendritic cells (DCs), which become partially activated. Using freshly isolated mouse DCs, we found that the interaction with SIgA was tissue and DC subtype dependent. Only DCs isolated from PPs and mesenteric lymph nodes interacted with the Ab. CD11c(+)CD11b(+) DCs internalized SIgA, while CD11c(+)CD19(+) DCs only bound SIgA on their surface, and no interaction occurred with CD11c(+)CD8alpha(+) DCs. We next examined whether SIgA could deliver a sizeable cargo to PP DCs in vivo by administering SIgA-Shigella flexneri immune complexes into a mouse ligated intestinal loop containing a PP. We found that such immune complexes entered the PPs and were internalized by subepithelial dome PP DCs, in contrast to S. flexneri alone that did not penetrate the intestinal epithelium in mice. Dissemination of intraepithelial S. flexneri delivered as immune complexes was limited to PPs and mesenteric lymph nodes. We propose that preexisting SIgA Abs associated with microbes contribute to mucosal defense by eliciting responses that prevent overreaction while maintaining productive immunity
Keywords
Animals/Antibodies/metabolism/Bacterial Translocation/immunology/Dendritic Cells/microbiology/Female/Immunoglobulin A,Secretory/physiology/Intestinal Mucosa/Mice/Mice,Inbred BALB C/Peyer's Patches/ultrastructure/Shigella flexneri
Pubmed
Web of science
Create date
19/02/2008 13:28
Last modification date
20/08/2019 15:36
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