High-intensity exercise in hypoxia improves endothelial function via increased nitric oxide bioavailability in C57BL/6 mice.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_7A430DC50D12
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
High-intensity exercise in hypoxia improves endothelial function via increased nitric oxide bioavailability in C57BL/6 mice.
Journal
Acta physiologica
Author(s)
Lavier J., Beaumann M., Menétrey S., Bouzourène K., Rosenblatt-Velin N., Pialoux V., Mazzolai L., Peyter A.C., Pellegrin M., Millet G.P.
ISSN
1748-1716 (Electronic)
ISSN-L
1748-1708
Publication state
Published
Issued date
10/2021
Peer-reviewed
Oui
Volume
233
Number
2
Pages
e13700
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The optimal exercise intensity to improve endothelial function remains unclear, as well as whether the addition of hypoxia could potentiate this function. Therefore, the aim of this study was to compare the effects of different exercise intensities in normoxia and hypoxia on vascular reactivity and nitric oxide (NO) bioavailability in mice.
C57BL/6 mice underwent treadmill running three times per week, for 4 weeks at either low, maximal or supramaximal intensity in normoxia or hypoxia (inspire oxygen fraction = 0.13). Vascular reactivity and expression of genes and proteins involved in NO production/bioavailability were assessed in aorta using isolated vessel tension experiments, RT-qPCR and western blot, respectively. Circulating NO metabolites and pro-/antioxidant markers were measured.
Hypoxic exercise improved both acetylcholine-induced vasorelaxation and phenylephrine-induced vasoconstriction compared to normoxic exercise, independently of intensity. In hypoxia, a higher acetylcholine-induced vasorelaxation was observed with high intensities (supramaximal and maximal) compared to low intensity. Exercise protocols modulated endothelial nitric oxide synthase (eNOS) and α1-adrenergic receptor (α <sub>1</sub> -AR) mRNA level, but not superoxide dismutase 3 (SOD3) and p47phox. No significant differences were observed for protein expression of α <sub>1</sub> -AR, total eNOS, phosphorylated eNOS, SOD isoforms and p47phox. However, plasma SOD and catalase activities were significantly increased in hypoxic supramaximal compared to hypoxic low intensity, while concentration of nitrotyrosine significantly decreased. The latter was also observed in hypoxic maximal and supramaximal compared to the same intensities in normoxia.
Hypoxic high-intensity exercise increases NO bioavailability and improves vascular function, opening promising clinical perspectives for cardiovascular disease prevention.
Keywords
Endothelial function, exercise training, high-intensity, hypoxia, nitric oxide bioavailability, endothelial function
Pubmed
Web of science
Open Access
Yes
Create date
15/06/2021 16:07
Last modification date
04/10/2021 6:38
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