Human gene MAGE-3 codes for an antigen recognized on a melanoma by autologous cytolytic T lymphocytes

Details

Serval ID
serval:BIB_79EDF8E5D0F4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Human gene MAGE-3 codes for an antigen recognized on a melanoma by autologous cytolytic T lymphocytes
Journal
Journal of Experimental Medicine
Author(s)
Gaugler  B., Van den Eynde  B., van der Bruggen  P., Romero  P., Gaforio  J. J., De Plaen  E., Lethe  B., Brasseur  F., Boon  T.
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
03/1994
Volume
179
Number
3
Pages
921-30
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 1
Abstract
Human melanoma cell line MZ2-MEL expresses several antigens recognized by autologous cytolytic T lymphocyte (CTL) clones. We reported previously the identification of a gene, named MAGE-1, that codes for one of these antigens named MZ2-E. We show here that antigen MZ2-D, which is present on the same tumor, is encoded by another member of the MAGE gene family named MAGE-3. Like MAGE-1, MAGE-3 is composed of three exons and the large open reading frame is entirely located in the third exon. Its sequence shows 73% identity with MAGE-1. Like MZ2-E, antigen MZ2-D is presented by HLA-A1. The antigenic peptide of MZ2-D is a nonapeptide that is encoded by the sequence of MAGE-3 that is homologous to the MAGE-1 sequence coding for the MZ2-E peptide. Competition experiments using single Ala-substituted peptides indicated that amino acid residues Asp in position 3 and Tyr in position 9 were essential for binding of the MAGE-1 peptide to HLA-A1. Gene MAGE-3 is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. It is expressed in a larger proportion of melanoma samples than MAGE-1. MAGE-3 encoded antigens may therefore have a wide applicability for specific immunotherapy of melanoma patients.
Keywords
Adult Amino Acid Sequence Antigens, Neoplasm/biosynthesis/*genetics Base Sequence Binding Sites Breast Neoplasms/metabolism Carcinoma, Squamous Cell/metabolism Cell Line Exons Female Fetus Genomic Library HLA-A1 Antigen/metabolism Head and Neck Neoplasms/metabolism Humans Lung Neoplasms/metabolism Male Melanoma/genetics/*metabolism Molecular Sequence Data *Neoplasm Proteins Oligodeoxyribonucleotides Open Reading Frames Organ Specificity T-Lymphocytes, Cytotoxic/*immunology Testis/metabolism Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:28
Last modification date
20/08/2019 14:36
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