Single-cell analyses identify bioengineered niches for enhanced maintenance of hematopoietic stem cells.

Details

Ressource 1Download: 28790449_BIB_7966BB1E2C7B.pdf (2431.00 [Ko])
State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_7966BB1E2C7B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Single-cell analyses identify bioengineered niches for enhanced maintenance of hematopoietic stem cells.
Journal
Nature communications
Author(s)
Roch A., Giger S., Girotra M., Campos V., Vannini N., Naveiras O., Gobaa S., Lutolf M.P.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
09/08/2017
Peer-reviewed
Oui
Volume
8
Number
1
Pages
221
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The in vitro expansion of long-term hematopoietic stem cells (HSCs) remains a substantial challenge, largely because of our limited understanding of the mechanisms that control HSC fate choices. Using single-cell multigene expression analysis and time-lapse microscopy, here we define gene expression signatures and cell cycle hallmarks of murine HSCs and the earliest multipotent progenitors (MPPs), and analyze systematically single HSC fate choices in culture. Our analysis revealed twelve differentially expressed genes marking the quiescent HSC state, including four genes encoding cell-cell interaction signals in the niche. Under basal culture conditions, most HSCs rapidly commit to become early MPPs. In contrast, when we present ligands of the identified niche components such as JamC or Esam within artificial niches, HSC cycling is reduced and long-term multipotency in vivo is maintained. Our approach to bioengineer artificial niches should be useful in other stem cell systems.Haematopoietic stem cell (HSC) self-renewal is not sufficiently understood to recapitulate in vitro. Here, the authors generate gene signature and cell cycle hallmarks of single murine HSCs, and use identified endothelial receptors Esam and JamC as substrates to enhance HSC growth in engineered niches.
Keywords
HPV, cervical cancer screening, human papillomavirus, mobile health, smartphone, visual approach
Pubmed
Web of science
Open Access
Yes
Create date
05/09/2017 13:11
Last modification date
29/04/2021 14:15
Usage data