Analysis of the enantiomers of citalopram and its demethylated metabolites using chiral liquid chromatography.
Details
Serval ID
serval:BIB_78BA074FA16B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of the enantiomers of citalopram and its demethylated metabolites using chiral liquid chromatography.
Journal
Journal of Chromatography. B, Biomedical Sciences and Applications
ISSN
1387-2273 (Print)
ISSN-L
1387-2273
Publication state
Published
Issued date
1998
Peer-reviewed
Oui
Volume
719
Number
1-2
Pages
234-238
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
A procedure using a chirobiotic V column is presented which allows separation of the enantiomers of citalopram and its two N-demethylated metabolites, and of the internal standard, alprenolol, in human plasma. Citalopram, demethylcitalopram and didemethylcitalopram, as well as the internal standard, were recovered from plasma by liquid-liquid extraction. The limits of quantification were found to be 5 ng/ml for each enantiomer of citalopram and demethylcitalopram, and 7.5 ng/ml for each enantiomer of didemethylcitalopram. Inter- and intra-day coefficients of variation varied from 2.4% to 8.6% for S- and R-citalopram, from 2.9% to 7.4% for S- and R-demethylcitalopram, and from 5.6% to 12.4% for S- and R- didemethylcitalopram. No interference was observed from endogenous compounds following the extraction of plasma samples from 10 different patients treated with citalopram. This method allows accurate quantification for each enantiomer and is, therefore, well suited for pharmacokinetic and drug interaction investigations. The presented method replaces a previously described highly sensitive and selective high-performance liquid chromatography procedure using an acetylated 3-cyclobond column which, because of manufactural problems, is no longer usable for the separation of the enantiomers of citalopram and its demethylated metabolites.
Keywords
Antidepressive Agents, Second-Generation/blood, Antidepressive Agents, Second-Generation/pharmacokinetics, Calibration, Chromatography, High Pressure Liquid/methods, Citalopram/blood, Citalopram/pharmacokinetics, Humans, Reproducibility of Results, Serotonin Uptake Inhibitors/blood, Serotonin Uptake Inhibitors/pharmacokinetics, Spectrometry, Fluorescence, Stereoisomerism
Pubmed
Web of science
Create date
01/03/2013 11:05
Last modification date
20/08/2019 14:35