The cell-end factor pom1p inhibits mid1p in specification of the cell division plane in fission yeast.

Details

Serval ID
serval:BIB_789F2F4787CF
Type
Article: article from journal or magazin.
Collection
Publications
Title
The cell-end factor pom1p inhibits mid1p in specification of the cell division plane in fission yeast.
Journal
Current Biology
Author(s)
Padte N.N., Martin S.G., Howard M., Chang F.
ISSN
0960-9822 (Print)
ISSN-L
0960-9822
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
16
Number
24
Pages
2480-2487
Language
english
Abstract
Intrinsic spatial cues ensure the proper placement of the cell division plane. In the fission yeast Schizosaccharomyces pombe, the position of the nucleus helps to direct the medial positioning of contractile-ring assembly and subsequent cell division . An important factor in this process is mid1p (anillin-like protein), which is a peripheral-membrane protein that forms a broad cortical band of dots overlying the nucleus in interphase and recruits myosin in early mitosis . How mid1p localizes to this cortical band and tracks the nucleus is not clear, especially because its localization is independent of the cytoskeleton . Here, we used a combination of experimental and computational approaches to test mid1p localization mechanisms. We provide evidence that pom1p, a DYRK-family protein kinase that forms a concentration gradient emanating from the nongrowing cell end, inhibits mid1p. In pom1 mutants, mid1p is distributed over half of the cell, covering the nongrowing cell end. This abnormal distribution is established in a dynamic manner in interphase and leads to the formation of misplaced or multiple contractile rings. Our computational and experimental results support a model in which both positive cues from the medial nucleus and negative cues from the cell tips specify the position of the division plane.
Keywords
Cell Division, Cell Nucleus/metabolism, Models, Theoretical, Protein Kinases/genetics, Protein Kinases/metabolism, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/metabolism, Protein-Tyrosine Kinases/genetics, Protein-Tyrosine Kinases/metabolism, Schizosaccharomyces/cytology, Schizosaccharomyces/genetics, Schizosaccharomyces pombe Proteins/genetics, Schizosaccharomyces pombe Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
18/03/2008 11:32
Last modification date
20/08/2019 14:35
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