Recovery of glucose-induced insulin secretion in a rat model of NIDDM is not accompanied by return of the B-cell GLUT2 glucose transporter.

Details

Serval ID
serval:BIB_7899A34207C9
Type
Article: article from journal or magazin.
Collection
Publications
Title
Recovery of glucose-induced insulin secretion in a rat model of NIDDM is not accompanied by return of the B-cell GLUT2 glucose transporter.
Journal
Diabetes
Author(s)
Chen C., Thorens B., Bonner-Weir S., Weir G.C., Leahy J.L.
ISSN
0012-1797[print], 0012-1797[linking]
Publication state
Published
Issued date
10/1992
Volume
41
Number
10
Pages
1320-1327
Language
english
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
The NSTZ rat model combines loss of glucose-induced insulin secretion with a reduced amount of the high Km B-cell glucose transporter, GLUT2. The purpose of this study was to determine whether the restoration of glucose-induced insulin secretion was paralleled by an increase of GLUT2. Rats injected at 2 days of age with 90 mg/kg STZ were studied at 8-13 wk of age. Insulin secretion was assessed in the isolated perfused pancreas with 16.7 mM glucose preceded by 40 min of 0 or 5.5 mM glucose. In control rats, 16.7 mM glucose caused the same large biphasic insulin response whether preceded by 0 or 5.5 mM glucose. In NSTZ rats, after 5.5 mM glucose, 16.7 mM glucose elicited virtually no rise in insulin release. In contrast, after 0 mM glucose, a large insulin response to the glucose challenge occurred that was equal to that of the control groups when the differences in B-cell mass were taken into account. However, the dose-response curve for glucose-induced insulin secretion was shifted to the left, and no second phase of insulin secretion was observed. GLUT2 was assessed after the perfusions by indirect immunofluorescence with anti-GLUT2 antisera. Both control groups showed homogenous staining in all B-cells. NSTZ rats perfused with 5.5 mM glucose had a marked diminution in GLUT2 staining. We observed no increase in GLUT2 staining in the NSTZ rats perfused with 0 mM glucose.(ABSTRACT TRUNCATED AT 250 WORDS)
Keywords
Animals, Blood Glucose/metabolism, Diabetes Mellitus, Experimental/metabolism, Diabetes Mellitus, Experimental/pathology, Diabetes Mellitus, Type 2/metabolism, Diabetes Mellitus, Type 2/pathology, Dose-Response Relationship, Drug, Glucose/pharmacology, Insulin/secretion, Islets of Langerhans/drug effects, Islets of Langerhans/pathology, Kinetics, Male, Monosaccharide Transport Proteins/metabolism, Pancreas/pathology, Perfusion, Rats, Rats, Sprague-Dawley, Reference Values
Pubmed
Web of science
Create date
24/01/2008 13:41
Last modification date
20/08/2019 14:35
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