Methods for integration site distribution analyses in animal cell genomes.

Details

Serval ID
serval:BIB_78620AD3F739
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Methods for integration site distribution analyses in animal cell genomes.
Journal
Methods
Author(s)
Ciuffi A., Ronen K., Brady T., Malani N., Wang G., Berry C.C., Bushman F.D.
ISSN
1095-9130 ([electronic])
Publication state
Published
Issued date
04/2009
Peer-reviewed
Oui
Volume
47
Number
4
Pages
261-268
Language
english
Abstract
The question of where retroviral DNA becomes integrated in chromosomes is important for understanding (i) the mechanisms of viral growth, (ii) devising new anti-retroviral therapy, (iii) understanding how genomes evolve, and (iv) developing safer methods for gene therapy. With the completion of genome sequences for many organisms, it has become possible to study integration targeting by cloning and sequencing large numbers of host-virus DNA junctions, then mapping the host DNA segments back onto the genomic sequence. This allows statistical analysis of the distribution of integration sites relative to the myriad types of genomic features that are also being mapped onto the sequence scaffold. Here we present methods for recovering and analyzing integration site sequences.
Keywords
retrovirus, integration site
Pubmed
Web of science
Create date
27/01/2010 17:02
Last modification date
20/08/2019 14:35
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