Heterogeneous Presynaptic Distribution of Munc13 Isoforms at Retinal Synapses and Identification of an Unconventional Bipolar Cell Type with Dual Expression of Munc13 Isoforms: A Study Using Munc13-EXFP Knock-in Mice.

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Serval ID
serval:BIB_785719A1AF7A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Heterogeneous Presynaptic Distribution of Munc13 Isoforms at Retinal Synapses and Identification of an Unconventional Bipolar Cell Type with Dual Expression of Munc13 Isoforms: A Study Using Munc13-EXFP Knock-in Mice.
Journal
International journal of molecular sciences
Author(s)
Gierke K., von Wittgenstein J., Hemmerlein M., Atorf J., Joachimsthaler A., Kremers J., Cooper B.H., Varoqueaux F., Regus-Leidig H., Brandstätter J.H.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Publication state
Published
Issued date
22/10/2020
Peer-reviewed
Oui
Volume
21
Number
21
Pages
E7848
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Munc13 isoforms are constituents of the presynaptic compartment of chemical synapses, where they govern important steps in preparing synaptic vesicles for exocytosis. The role of Munc13-1, -2 and -3 is well documented in brain neurons, but less is known about their function and distribution among the neurons of the retina and their conventional and ribbon-type chemical synapses. Here, we examined the retinae of Munc13-1-, -2-, and -3-EXFP knock-in (KI) mice with a combination of immunocytochemistry, physiology, and electron microscopy. We show that knock-in of Munc13-EXFP fusion proteins did not affect overall retinal anatomy or synapse structure, but slightly affected synaptic transmission. By labeling Munc13-EXFP KI retinae with specific antibodies against Munc13-1, -2 and -3, we found that unlike in the brain, most retinal synapses seem to operate with a single Munc13 isoform. A surprising exception to this rule was type 6 ON bipolar cells, which expressed two Munc13 isoforms in their synaptic terminals, ubMunc13-2 and Munc13-3. The results of this study provide an important basis for future studies on the contribution of Munc13 isoforms in visual signal processing in the mammalian retina.
Keywords
Animals, Electroretinography, Female, Intracellular Signaling Peptides and Proteins/genetics, Intracellular Signaling Peptides and Proteins/metabolism, Male, Mice, Inbred C57BL, Mice, Transgenic, Nerve Tissue Proteins/genetics, Nerve Tissue Proteins/metabolism, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/immunology, Recombinant Fusion Proteins/metabolism, Retina/cytology, Retina/physiology, Retina/ultrastructure, Synapses/physiology, Synaptic Transmission/physiology, Munc13-1, Munc13-3, brMunc13-2, conventional synapse, retina, ribbon synapse, type 6 ON bipolar cell, ubMunc13-2
Pubmed
Web of science
Open Access
Yes
Create date
02/11/2020 12:45
Last modification date
08/08/2024 6:35
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