Distinct patterns of skeletal muscle mitochondria fusion, fission and mitophagy upon duration of exercise training.

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Serval ID
serval:BIB_77F45673CA1F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Distinct patterns of skeletal muscle mitochondria fusion, fission and mitophagy upon duration of exercise training.
Journal
Acta physiologica
Author(s)
Arribat Y., Broskey N.T., Greggio C., Boutant M., Conde Alonso S., Kulkarni S.S., Lagarrigue S., Carnero E.A., Besson C., Cantó C., Amati F.
ISSN
1748-1716 (Electronic)
ISSN-L
1748-1708
Publication state
Published
Issued date
02/2019
Peer-reviewed
Oui
Volume
225
Number
2
Pages
e13179
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Healthy ageing interventions encompass regular exercise to prevent mitochondrial dysfunction, key player in sarcopenia pathogenesis. Mitochondrial biogenesis has been well documented, but mitochondrial remodelling in response to exercise training is poorly understood. Here we investigated fusion, fission and mitophagy before and after an exercise intervention in older adults.
Skeletal muscle biopsies were collected from 22 healthy sedentary men and women before and after 4 months of supervised training. Eight lifelong trained age- and gender-matched volunteers served as positive controls. Transmission electron microscopy was used to estimate mitochondrial content. Western blotting and qRT-PCR were used to detect changes in specific proteins and transcripts.
After intervention, mitochondrial content increased to levels of controls. While enhancement of fusion was prevalent after intervention, inhibition of fission and increased mitophagy were dominant in controls. Similarly to PARKIN, BCL2L13 content was higher in controls. The observed molecular adaptations paralleled long-term effects of training on physical fitness, exercise efficiency and oxidative capacity.
This study describes distinct patterns of molecular adaptations in human skeletal muscle under chronic exercise training. After 16 weeks of exercise, the pattern was dominated by fusion to increase mitochondrial content to the metabolic demands of exercise. In lifelong exercise, the pattern was dominated by mitophagy synchronized with increased fusion and decreased fission, indicating an increased mitochondrial turnover. In addition to these temporally distinct adaptive mechanisms, this study suggests for the first time a specific role of BCL2L13 in chronic exercise that requires constant maintenance of mitochondrial quality.
Keywords
Adaptation, Physiological, Aged, Case-Control Studies, Exercise, Female, Humans, Male, Mitochondria/pathology, Mitochondrial Dynamics, Mitophagy, Muscle, Skeletal/physiopathology, DRP1, MFN2, PINK1, chronic exercise, mitochondria dynamics, skeletal muscle
Pubmed
Web of science
Open Access
Yes
Funding(s)
Swiss National Science Foundation / Careers / PZ00P3-126339
Swiss National Science Foundation / Careers / PZ00P3-149398
Swiss National Science Foundation / Projects / 320030_170062
Create date
03/09/2018 13:53
Last modification date
06/03/2020 6:20
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