Lipid Internal Dynamics Probed in Nanodiscs.

Details

Serval ID
serval:BIB_77A89DB22B7B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Lipid Internal Dynamics Probed in Nanodiscs.
Journal
Chemphyschem
Author(s)
Martinez D., Decossas M., Kowal J., Frey L., Stahlberg H., Dufourc E.J., Riek R., Habenstein B., Bibow S., Loquet A.
ISSN
1439-7641 (Electronic)
ISSN-L
1439-4235
Publication state
Published
Issued date
06/10/2017
Peer-reviewed
Oui
Volume
18
Number
19
Pages
2651-2657
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Nanodiscs offer a very promising tool to incorporate membrane proteins into native-like lipid bilayers and an alternative to liposomes to maintain protein functions and protein-lipid interactions in a soluble nanoscale object. The activity of the incorporated membrane protein appears to be correlated to its dynamics in the lipid bilayer and by protein-lipid interactions. These two parameters depend on the lipid internal dynamics surrounded by the lipid-encircling discoidal scaffold protein that might differ from more unrestricted lipid bilayers observed in vesicles or cellular extracts. A solid-state NMR spectroscopy investigation of lipid internal dynamics and thermotropism in nanodiscs is reported. The gel-to-fluid phase transition is almost abolished for nanodiscs, which maintain lipid fluid properties for a large temperature range. The addition of cholesterol allows fine-tuning of the internal bilayer dynamics by increasing chain ordering. Increased site-specific order parameters along the acyl chain reflect a higher internal ordering in nanodiscs compared with liposomes at room temperature; this is induced by the scaffold protein, which restricts lipid diffusion in the nanodisc area.
Keywords
Deuterium, Lipid Bilayers/chemistry, Lipids/chemistry, Magnetic Resonance Spectroscopy, Membrane Proteins/chemistry, Nanostructures/chemistry, Thermodynamics, NMR spectroscopy, lipids, membranes, nanostructures, solid-state structures
Pubmed
Web of science
Open Access
Yes
Create date
09/06/2023 16:02
Last modification date
08/07/2023 6:50
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