Lipid Internal Dynamics Probed in Nanodiscs.
Details
Serval ID
serval:BIB_77A89DB22B7B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Lipid Internal Dynamics Probed in Nanodiscs.
Journal
Chemphyschem
ISSN
1439-7641 (Electronic)
ISSN-L
1439-4235
Publication state
Published
Issued date
06/10/2017
Peer-reviewed
Oui
Volume
18
Number
19
Pages
2651-2657
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Nanodiscs offer a very promising tool to incorporate membrane proteins into native-like lipid bilayers and an alternative to liposomes to maintain protein functions and protein-lipid interactions in a soluble nanoscale object. The activity of the incorporated membrane protein appears to be correlated to its dynamics in the lipid bilayer and by protein-lipid interactions. These two parameters depend on the lipid internal dynamics surrounded by the lipid-encircling discoidal scaffold protein that might differ from more unrestricted lipid bilayers observed in vesicles or cellular extracts. A solid-state NMR spectroscopy investigation of lipid internal dynamics and thermotropism in nanodiscs is reported. The gel-to-fluid phase transition is almost abolished for nanodiscs, which maintain lipid fluid properties for a large temperature range. The addition of cholesterol allows fine-tuning of the internal bilayer dynamics by increasing chain ordering. Increased site-specific order parameters along the acyl chain reflect a higher internal ordering in nanodiscs compared with liposomes at room temperature; this is induced by the scaffold protein, which restricts lipid diffusion in the nanodisc area.
Keywords
Deuterium, Lipid Bilayers/chemistry, Lipids/chemistry, Magnetic Resonance Spectroscopy, Membrane Proteins/chemistry, Nanostructures/chemistry, Thermodynamics, NMR spectroscopy, lipids, membranes, nanostructures, solid-state structures
Pubmed
Web of science
Open Access
Yes
Create date
09/06/2023 16:02
Last modification date
08/07/2023 6:50