Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2

Details

Serval ID
serval:BIB_774077149E48
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2
Journal
Cancer Research
Author(s)
Suardet  L., Gaide  A. C., Calmes  J. M., Sordat  B., Givel  J. C., Eliason  J. F., Odartchenko  N.
ISSN
0008-5472 (Print)
Publication state
Published
Issued date
07/1992
Volume
52
Number
13
Pages
3705-12
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 1
Abstract
We have established 3 new human colorectal cancer cell lines (LS411N, LS513, and LS1034) from clinical biopsy samples. These lines are tumorigenic and grow s.c. as adenocarcinomas in nude mouse xenografts. Specific marker chromosomes are observed in each line. Carcinoembryonic antigen is expressed at the surface of all 3 lines, but with marked quantitative differences. Indeed, less than 10% of the cells from the HT-29 line used as a reference express carcinoembryonic antigen while more than 90% of the LS1034 cells do so. LS513 and LS1034 consistently express HLA class I antigens and intercellular adhesion molecule 1 which are not detected at the surface of the LS411N cells. No expression of HLA class II antigens DR, DQ, and DP has been measured on any of the lines. All three lines grow well in 5% fetal calf serum medium without addition of exogenous growth factors. The LS1034 line has been adapted to growth in serum-free conditions and exhibits increased clonogenicity when cells are seeded in serum-free methylcellulose medium, as compared with medium containing 5% fetal calf serum. The LS513 and LS1034 lines have proved to be of particular interest since they respond to the growth-inhibitory action of TGF-beta 1 and TGF-beta 2 in both liquid and semisolid medium. Both factors were, at pM concentrations, equipotent inhibitors of LS1034 cell proliferation. In contrast, higher concentrations of TGF-beta 1 are inhibitory for proliferation of LS513 cells, whereas TGF-beta 2 has no effect on the growth of these cells in liquid assay. On this basis, using appropriate anti-TGF-beta 1 and anti-TGF-beta 1 IgY, we developed a bioassay for TGF-beta 1 and TGF-beta 2. Two of the three lines have indeed been shown to produce latent-TGF-beta 1 activity.
Keywords
Adult Antigens, Surface/analysis Colorectal Neoplasms/genetics/immunology/*pathology Humans Karyotyping Male Middle Aged Neoplasm Transplantation Transforming Growth Factor beta/analysis/*pharmacology Transplantation, Heterologous Tumor Cells, Cultured/drug effects
Pubmed
Web of science
Create date
28/01/2008 8:56
Last modification date
20/08/2019 14:34
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