Vaccinia virus produces late mRNAs by discontinuous synthesis.

Details

Serval ID
serval:BIB_77375F9948BE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Vaccinia virus produces late mRNAs by discontinuous synthesis.
Journal
Cell
Author(s)
Bertholet C., Van Meir E., ten Heggeler-Bordier B., Wittek R.
ISSN
0092-8674[print], 0092-8674[linking]
Publication state
Published
Issued date
07/1987
Volume
50
Number
2
Pages
153-162
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
We describe the unusual structure of a vaccinia virus late mRNA. In these molecules, the protein-coding sequences of a major late structural polypeptide are preceded by long leader RNAs, which in some cases are thousands of nucleotides long. These sequences map to different regions of the viral genome and in one instance are separated from the late gene by more than 100 kb of DNA. Moreover, the leader sequences map either upstream or downstream of the late gene, are transcribed from either DNA strand, and are fused to the late gene coding sequence via a poly(A) stretch. This demonstrates that vaccinia virus produces late mRNAs by tagging the protein-coding sequences onto the 3' end of other RNAs.
Keywords
Base Sequence, Cloning, Molecular, DNA/metabolism, DNA Restriction Enzymes, DNA, Viral/genetics, Genes, Viral, Hela Cells/metabolism, Humans, Molecular Weight, Nucleic Acid Hybridization, RNA, Messenger/biosynthesis, RNA, Messenger/genetics, Vaccinia virus/genetics
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 14:34
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