Evaluation of the interactions between multiwalled carbon nanotubes and caco-2 cells

Details

Serval ID
serval:BIB_76FD2EF38AD2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Evaluation of the interactions between multiwalled carbon nanotubes and caco-2 cells
Journal
Journal of Toxicology and Environmental Health. Part A
Author(s)
Clark Katherine A., O'Driscoll Cliona, Cooke Carol A., Smith Bill A., Wepasnick Kevin, Fairbrother D. Howard , Lees Peter S.J., Bressler Joseph P.
ISSN
1528-7394
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
75
Number
1
Pages
25-35
Language
english
Abstract
The aim of this study was to determine whether multiwalled carbon nanotubes (MWNCT) are taken up by and are toxic to human intestinal enterocytes using the Caco-2 cell model. Caco-2 cells were exposed to 50 ?g/ml MWCNT (oxidized or pristine) for 24 h, and experiments were repeated in the presence of 2.5 mg/L natural organic matter. Cells displayed many of the properties that characterize enterocytes, such as apical microvilli, basolateral basement membrane, and glycogen. The cell monolayers also displayed tight junctions and electrical resistance. Exposure to pristine and oxidized MWCNT, with or without natural organic matter, did not markedly affect viability, which was assessed by measuring activity of released lactate dehydrogenase (LDH) and staining with propidium iodide. Ultrastructural analysis revealed some damage to microvilli colocalized with the MWCNT; however, neither type of MWCNT was taken up by Caco-2 cells. In contrast, pristine and oxidized MWCNT were taken up by the macrophage RAW 264.7 line. Our study suggests that intestinal enterocytes cells do not take up MWCNT. [Authors]
Keywords
Nanotubes, Carbon , Enterocytes , Caco-2 Cells ,
Pubmed
Web of science
Create date
20/02/2012 16:27
Last modification date
20/08/2019 14:34
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