Endothelial Colony-Forming Cells Dysfunctions Are Associated with Arterial Hypertension in a Rat Model of Intrauterine Growth Restriction.

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License: CC BY 4.0
Serval ID
serval:BIB_76C775F89D99
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Endothelial Colony-Forming Cells Dysfunctions Are Associated with Arterial Hypertension in a Rat Model of Intrauterine Growth Restriction.
Journal
International journal of molecular sciences
Author(s)
Simoncini S., Coppola H., Rocca A., Bachmann I., Guillot E., Zippo L., Dignat-George F., Sabatier F., Bedel R., Wilson A., Rosenblatt-Velin N., Armengaud J.B., Menétrey S., Peyter A.C., Simeoni U., Yzydorczyk C.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Publication state
Published
Issued date
21/09/2021
Peer-reviewed
Oui
Volume
22
Number
18
Pages
10159
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Infants born after intrauterine growth restriction (IUGR) are at risk of developing arterial hypertension at adulthood. The endothelium plays a major role in the pathogenesis of hypertension. Endothelial colony-forming cells (ECFCs), critical circulating components of the endothelium, are involved in vasculo-and angiogenesis and in endothelium repair. We previously described impaired functionality of ECFCs in cord blood of low-birth-weight newborns. However, whether early ECFC alterations persist thereafter and could be associated with hypertension in individuals born after IUGR remains unknown. A rat model of IUGR was induced by a maternal low-protein diet during gestation versus a control (CTRL) diet. In six-month-old offspring, only IUGR males have increased systolic blood pressure (tail-cuff plethysmography) and microvascular rarefaction (immunofluorescence). ECFCs isolated from bone marrow of IUGR versus CTRL males displayed a decreased proportion of CD31+ versus CD146+ staining on CD45- cells, CD34 expression (flow cytometry, immunofluorescence), reduced proliferation (BrdU incorporation), and an impaired capacity to form capillary-like structures (Matrigel test), associated with an impaired angiogenic profile (immunofluorescence). These dysfunctions were associated with oxidative stress (increased superoxide anion levels (fluorescent dye), decreased superoxide dismutase protein expression, increased DNA damage (immunofluorescence), and stress-induced premature senescence (SIPS; increased beta-galactosidase activity, increased p16 <sup>INK4a</sup> , and decreased sirtuin-1 protein expression). This study demonstrated an impaired functionality of ECFCs at adulthood associated with arterial hypertension in individuals born after IUGR.
Keywords
arterial hypertension, developmental programming, endothelial colony-forming cells, intrauterine growth restriction, oxidative stress, stress-induced premature senescence
Pubmed
Web of science
Open Access
Yes
Create date
04/10/2021 10:59
Last modification date
12/01/2022 8:11
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