Glutamate exocytosis from astrocytes controls synaptic strength.

Details

Serval ID
serval:BIB_769F4B538F36
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Glutamate exocytosis from astrocytes controls synaptic strength.
Journal
Nature Neuroscience
Author(s)
Jourdain P., Bergersen L.H., Bhaukaurally K., Bezzi P., Santello M., Domercq M., Matute C., Tonello F., Gundersen V., Volterra A.
ISSN
1097-6256 (Print)
ISSN-L
1097-6256
Publication state
Published
Issued date
2007
Volume
10
Number
3
Pages
331-339
Language
english
Abstract
The release of transmitters from glia influences synaptic functions. The modalities and physiological functions of glial release are poorly understood. Here we show that glutamate exocytosis from astrocytes of the rat hippocampal dentate molecular layer enhances synaptic strength at excitatory synapses between perforant path afferents and granule cells. The effect is mediated by ifenprodil-sensitive NMDA ionotropic glutamate receptors and involves an increase of transmitter release at the synapse. Correspondingly, we identify NMDA receptor 2B subunits on the extrasynaptic portion of excitatory nerve terminals. The receptor distribution is spatially related to glutamate-containing synaptic-like microvesicles in the apposed astrocytic processes. This glial regulatory pathway is endogenously activated by neuronal activity-dependent stimulation of purinergic P2Y1 receptors on the astrocytes. Thus, we provide the first combined functional and ultrastructural evidence for a physiological control of synaptic activity via exocytosis of glutamate from astrocytes.
Keywords
Analysis of Variance, Animals, Astrocytes/drug effects, Astrocytes/metabolism, Electric Stimulation/methods, Excitatory Amino Acid Agonists/pharmacology, Excitatory Amino Acid Antagonists/pharmacology, Exocytosis/drug effects, Exocytosis/physiology, Glutamic Acid/metabolism, Hippocampus/cytology, Membrane Potentials/drug effects, Membrane Potentials/physiology, Microscopy, Immunoelectron/methods, N-Methylaspartate/pharmacology, Neurons/drug effects, Neurons/physiology, Patch-Clamp Techniques/methods, Perforant Pathway/physiology, Perforant Pathway/radiation effects, Piperidines/pharmacology, Rats, Rats, Sprague-Dawley, Receptors, N-Methyl-D-Aspartate/metabolism, Receptors, N-Methyl-D-Aspartate/ultrastructure, Synapses/metabolism, Synapses/ultrastructure, Synaptic Transmission/physiology
Pubmed
Web of science
Create date
24/01/2008 15:37
Last modification date
20/08/2019 15:33
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