RecA-mediated strand exchange traverses substitutional heterologies more easily than deletions or insertions.

Details

Serval ID
serval:BIB_769B9002A891
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
RecA-mediated strand exchange traverses substitutional heterologies more easily than deletions or insertions.
Journal
Nucleic Acids Research
Author(s)
Bucka A., Stasiak A.
ISSN
1362-4962[electronic], 0305-1048[linking]
Publication state
Published
Issued date
06/2001
Volume
29
Number
12
Pages
2464-2470
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
RecA protein in bacteria and its eukaryotic homolog Rad51 protein are responsible for initiation of strand exchange between homologous DNA molecules. This process is crucial for homologous recombination, the repair of certain types of DNA damage and for the reinitiation of DNA replication on collapsed replication forks. We show here, using two different types of in vitro assays, that in the absence of ATP hydrolysis RecA-mediated strand exchange traverses small substitutional heterologies between the interacting DNAs, whereas small deletions or insertions block the ongoing strand exchange. We discuss evolutionary implications of RecA selectivity against insertions and deletions and propose a molecular mechanism by which RecA can exert this selectivity.
Keywords
Adenosine Triphosphate/metabolism, Adenylyl Imidodiphosphate/metabolism, Artifacts, DNA Repair/genetics, DNA Replication/genetics, DNA, Single-Stranded/chemistry, DNA, Single-Stranded/genetics, Escherichia coli/enzymology, Escherichia coli/genetics, Hydrolysis, Models, Biological, Mutagenesis, Insertional/genetics, Nucleic Acid Conformation, Oligodeoxyribonucleotides/chemistry, Oligodeoxyribonucleotides/genetics, Rec A Recombinases/metabolism, Recombination, Genetic/genetics, Sequence Deletion/genetics, Substrate Specificity
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 10:36
Last modification date
20/08/2019 14:33
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