Genome Sequence of the Wheat Stem Sawfly, Cephus cinctus, Representing an Early-Branching Lineage of the Hymenoptera, Illuminates Evolution of Hymenopteran Chemoreceptors.

Details

Ressource 1Download: evy232.pdf (738.19 [Ko])
State: Serval
Version: Final published version
Serval ID
serval:BIB_768CA970E2F8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Genome Sequence of the Wheat Stem Sawfly, Cephus cinctus, Representing an Early-Branching Lineage of the Hymenoptera, Illuminates Evolution of Hymenopteran Chemoreceptors.
Journal
Genome biology and evolution
Author(s)
Robertson H.M., Waterhouse R.M., Walden KKO, Ruzzante L., Reijnders MJMF, Coates B.S., Legeai F., Gress J.C., Biyiklioglu S., Weaver D.K., Wanner K.W., Budak H.
ISSN
1759-6653 (Electronic)
ISSN-L
1759-6653
Publication state
Published
Issued date
01/11/2018
Peer-reviewed
Oui
Volume
10
Number
11
Pages
2997-3011
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: epublish
Abstract
The wheat stem sawfly, Cephus cinctus, is a major pest of wheat and key ecological player in the grasslands of western North America. It also represents the distinctive Cephoidea superfamily of sawflies (Symphyta) that appeared early during the hymenopteran radiation, but after three early-branching eusymphytan superfamilies that form the base of the order Hymenoptera. We present a high-quality draft genome assembly of 162 Mb in 1,976 scaffolds with a scaffold N50 of 622 kb. Automated gene annotation identified 11,210 protein-coding gene models and 1,307 noncoding RNA models. Thirteen percent of the assembly consists of ∼58,000 transposable elements partitioned equally between Class-I and Class-II elements. Orthology analysis reveals that 86% of Cephus proteins have identifiable orthologs in other insects. Phylogenomic analysis of conserved subsets of these proteins supports the placement of the Cephoidea between the Eusymphyta and the parasitic woodwasp superfamily Orussoidea. Manual annotation and phylogenetic analysis of families of odorant, gustatory, and ionotropic receptors, plus odorant-binding proteins, shows that Cephus has representatives for most conserved and expanded gene lineages in the Apocrita (wasps, ants, and bees). Cephus has also maintained several insect gene lineages that have been lost from the Apocrita, most prominently the carbon dioxide receptor subfamily. Furthermore, Cephus encodes a few small lineage-specific chemoreceptor gene family expansions that might be involved in adaptations to new grasses including wheat. These comparative analyses identify gene family members likely to have been present in the hymenopteran ancestor and provide a new perspective on the evolution of the chemosensory gene repertoire.
Keywords
Animals, DNA Transposable Elements, Evolution, Molecular, Female, Genome, Insect, Hymenoptera/genetics, Insect Proteins/genetics, Male, MicroRNAs/genetics, Phylogeny, Receptors, Odorant/genetics
Pubmed
Web of science
Open Access
Yes
Create date
29/10/2018 15:22
Last modification date
08/05/2019 20:34
Usage data