Epistasis-driven identification of SLC25A51 as a regulator of human mitochondrial NAD import.

Details

Serval ID
serval:BIB_75C6C477E13D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Epistasis-driven identification of SLC25A51 as a regulator of human mitochondrial NAD import.
Journal
Nature communications
Author(s)
Girardi E., Agrimi G., Goldmann U., Fiume G., Lindinger S., Sedlyarov V., Srndic I., Gürtl B., Agerer B., Kartnig F., Scarcia P., Di Noia M.A., Liñeiro E., Rebsamen M., Wiedmer T., Bergthaler A., Palmieri L., Superti-Furga G.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
01/12/2020
Peer-reviewed
Oui
Volume
11
Number
1
Pages
6145
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
About a thousand genes in the human genome encode for membrane transporters. Among these, several solute carrier proteins (SLCs), representing the largest group of transporters, are still orphan and lack functional characterization. We reasoned that assessing genetic interactions among SLCs may be an efficient way to obtain functional information allowing their deorphanization. Here we describe a network of strong genetic interactions indicating a contribution to mitochondrial respiration and redox metabolism for SLC25A51/MCART1, an uncharacterized member of the SLC25 family of transporters. Through a combination of metabolomics, genomics and genetics approaches, we demonstrate a role for SLC25A51 as enabler of mitochondrial import of NAD, showcasing the potential of genetic interaction-driven functional gene deorphanization.
Keywords
Biological Transport, Epistasis, Genetic, Humans, Mitochondria/genetics, Mitochondria/metabolism, NAD/metabolism, Oxidation-Reduction, Uncoupling Protein 1/genetics, Uncoupling Protein 1/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
07/02/2022 11:01
Last modification date
08/02/2022 7:37
Usage data