Exonic sequences are required for elicitor and light activation of a plant defense gene, but promoter sequences are sufficient for tissue specific expression

Details

Serval ID
serval:BIB_758B439F4DAB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Exonic sequences are required for elicitor and light activation of a plant defense gene, but promoter sequences are sufficient for tissue specific expression
Journal
EMBO Journal
Author(s)
Douglas  C. J., Hauffe  K. D., Ites-Morales  M. E., Ellard  M., Paszkowski  U., Hahlbrock  K., Dangl  J. L.
ISSN
0261-4189
Publication state
Published
Issued date
07/1991
Peer-reviewed
Oui
Volume
10
Number
7
Pages
1767-75
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: Jul
Abstract
The parsley 4CL-1 gene encodes 4-coumarate:CoA ligase, a key enzyme of general phenylpropanoid metabolism. As well as being transcriptionally activated by such stresses as pathogen infection, UV-irradiation, and wounding, expression of 4CL-1 is developmentally regulated. In this paper we present evidence that 4CL-1 cis-acting elements which control stress-induced and developmental expression are physically separated. The ability of a series of 4CL gene constructions to respond to elicitor and light in stably or transiently transformed parsley cells was tested. While inducible expression was observed from all templates in which the 4CL-1 structural gene was fused to the 4CL-1 promoter, fusions of the promoter to the GUS reporter gene were completely unresponsive. The element(s) required for responsiveness appear to be exonic, since 4CL-1 introns and 3' flanking DNA had no effect on inducibility. Furthermore, this unconventional regulatory mode operates in transgenic tobacco plants, where we show that a 4CL-1 promoter fragment specifies correct cell-specific expression when fused to GUS yet is unresponsive to elicitor and light.
Keywords
Base Sequence Coenzyme A Ligases/biosynthesis/genetics Enhancer Elements (Genetics)/radiation effects *Exons *Gene Expression Regulation Light Molecular Sequence Data Plants/enzymology/*genetics Plants, Toxic *Promoter Regions (Genetics) Recombinant Proteins/biosynthesis/genetics Tobacco/enzymology/genetics Transformation, Genetic Ultraviolet Rays
Pubmed
Web of science
Create date
24/01/2008 19:47
Last modification date
20/08/2019 14:32
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