Implementation and evaluation of methods for the optimal detection of carbapenem-resistant and colistin-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from stools.
Details
Serval ID
serval:BIB_75797BC41BD4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Implementation and evaluation of methods for the optimal detection of carbapenem-resistant and colistin-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from stools.
Journal
Diagnostic microbiology and infectious disease
ISSN
1879-0070 (Electronic)
ISSN-L
0732-8893
Publication state
Published
Issued date
10/2020
Peer-reviewed
Oui
Volume
98
Number
2
Pages
115121
Language
english
Notes
Publication types: Evaluation Study ; Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Carbapenem-resistant Acinetobacter baumannii (CRAB) and Pseudomonas aeruginosa (CRPA), as well as polymyxin-resistant A. baumannii (PMR-AB) and P. aeruginosa (PMR-PA), were used to test different enrichment strategies from spiked stools. Three procedures were compared, namely, direct inoculation on selective plates and plating after a 24-h enrichment step in tryptic soy broth with or without antibiotics. Selective agar plates were used including CHROMagar-Pseudomonas supplemented with meropenem (2 mg/L), and CHROMagar-MDR-Acinetobacter agar and CHROMagar COL-APSE plates. Use of enrichment broths significantly enhanced the recovery of CRAB, CRPA, PMR-AB, and PMR-PA. However, supplementing or not the pre-enrichment broth with antibiotics had no impact. The proposed strategy for screening multidrug-resistant nonfermenters is of low cost, is easy to implement, and might be useful for outbreak containment.
Keywords
Acinetobacter Infections/diagnosis, Acinetobacter Infections/microbiology, Acinetobacter baumannii/drug effects, Acinetobacter baumannii/growth & development, Acinetobacter baumannii/isolation & purification, Anti-Bacterial Agents/pharmacology, Bacteriological Techniques/methods, Carbapenems/pharmacology, Colistin/pharmacology, Culture Media, Drug Resistance, Multiple, Bacterial, Feces/microbiology, Humans, Microbial Sensitivity Tests, Pseudomonas Infections/diagnosis, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/drug effects, Pseudomonas aeruginosa/growth & development, Pseudomonas aeruginosa/isolation & purification, Sensitivity and Specificity, Carbapenem, Colistin, Enrichment, Optimal, Stools
Pubmed
Web of science
Open Access
Yes
Create date
24/07/2020 14:05
Last modification date
13/04/2024 7:13