Neuropeptide Y secretion from a human insulinoma.

Details

Serval ID
serval:BIB_7569F8082640
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Title
Neuropeptide Y secretion from a human insulinoma.
Journal
Journal of endocrinological investigation
Author(s)
Waeber G., Hurlimann J., Haefliger J.A., Gomez F., Nicod P., Grouzmann E.
ISSN
0391-4097
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
19
Number
3
Pages
190-5
Language
english
Notes
Publication types: Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
Neuropeptide Y (NPY) is a 36 aminoacid peptide known to inhibit glucose-stimulated insulin secretion. NPY has been shown to be synthesized and secreted by rat islets of Langerhans. More recently, we described the presence on NPY within human islets of Langerhans and in several pancreatic endocrine tumors. In this report, we describe the case of a patient presenting with an insulinoma who underwent the surgical resection of the tumor and was studied in vivo and in vitro for NPY production. Using a highly specific and sensitive two-site amplified enzyme-linked immunosorbent assay, we detected high plasma NPY levels in the patient prior to the surgical resection of the tumor which returned to normal after surgery. NPY was secreted from the tumor when kept in primary cell culture. Furthermore, immunohistochemistry of the insulinoma revealed the presence of NPY and its C-flanking peptide together with insulin, chromogranin and neuron specific enolase. It is concluded that elevated circulating NPY levels observed in this patient with an insulinoma reflected in vivo secretion by the tumor and it is hypothesized that NPY could potentially be used as an endocrine marker in patients with suspected insulinoma.
Keywords
Aged, Aged, 80 and over, Chromogranins, Female, Humans, Immunoenzyme Techniques, Immunohistochemistry, Insulin, Insulinoma, Neuropeptide Y, Pancreatic Neoplasms, Phosphopyruvate Hydratase, Tumor Cells, Cultured
Pubmed
Web of science
Create date
25/01/2008 14:48
Last modification date
20/08/2019 15:32
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