Insulin-like growth factor I (IGF I) stimulates DNA synthesis in fetal rat brain cell cultures.

Details

Serval ID
serval:BIB_7541D49B3745
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Insulin-like growth factor I (IGF I) stimulates DNA synthesis in fetal rat brain cell cultures.
Journal
Developmental Brain Research
Author(s)
Lenoir D., Honegger P.
ISSN
0006-8993 (Print)
ISSN-L
0006-8993
Publication state
Published
Issued date
1983
Volume
7
Number
2-3
Pages
205-213
Language
english
Abstract
Addition of insulin, IGF I or IGF II to serum-free cultures of fetal rat brain cells (gestation day 15/16) significantly stimulates DNA synthesis. The dose-response curves show that IGF I is more potent than insulin; half maximal stimulation of [3H]thymidine incorporation is obtained at about 0.4 nM IGF I and 14 nM insulin, respectively. Cultures initiated 2 days later (gestation day 17/18) showed a decreased responsiveness to both peptides. No additive effect was observed after combined addition of both peptides at near-maximal doses. Both peptides show a latency of action of about 12-18 h. In the presence of either IGF or insulin, neuronal as well as glial enzymes are increased, suggesting that neuronal and glial precursor cell division is influenced. IGF I and IGF II interact with a specific binding site for which insulin competes very weakly; however IGF I and IGF II bind with relatively high affinity to the insulin specific binding site. The present results support the hypothesis that both insulin and IGF stimulate mitotic activity by interacting with specific somatomedin receptors and suggest a physiological role of IGF in the developing brain.
Keywords
Animals, Brain/cytology, Brain/drug effects, Cell Differentiation/drug effects, Culture Techniques, DNA/biosynthesis, Dose-Response Relationship, Drug, Female, Fetus, Insulin/metabolism, Insulin/pharmacology, Neurons/cytology, Neurotransmitter Agents/metabolism, Peptides/metabolism, Peptides/pharmacology, Pregnancy, Rats, Rats, Inbred Strains, Receptors, Cell Surface/metabolism, Receptors, Somatomedin, Somatomedins/metabolism, Somatomedins/pharmacology, Thymidine/metabolism
Pubmed
Web of science
Create date
24/01/2008 13:11
Last modification date
20/08/2019 14:32
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