Mesenchymal stem cells support expansion of in vitro irradiated CD34(+) cells in the presence of SCF, FLT3 ligand, TPO and IL3: potential application to autologous cell therapy in accidentally irradiated victims

Details

Serval ID
serval:BIB_75412D15034B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Mesenchymal stem cells support expansion of in vitro irradiated CD34(+) cells in the presence of SCF, FLT3 ligand, TPO and IL3: potential application to autologous cell therapy in accidentally irradiated victims
Journal
Radiat Res
Author(s)
Mourcin F., Grenier N., Mayol J. F., Lataillade J. J., Sotto J. J., Herodin F., Drouet M.
ISSN
0033-7587 (Print)
ISSN-L
0033-7587
Publication state
Published
Issued date
07/2005
Volume
164
Number
1
Pages
1-9
Language
english
Notes
Mourcin, Frederic
Grenier, Nancy
Mayol, Jean-Francois
Lataillade, Jean-Jacques
Sotto, Jean-Jacques
Herodin, Francis
Drouet, Michel
eng
Research Support, Non-U.S. Gov't
2005/06/22
Radiat Res. 2005 Jul;164(1):1-9. doi: 10.1667/rr3384.
Abstract
Ex vivo expansion of residual autologous hematopoietic stem and progenitor cells collected from victims soon after accidental irradiation (autologous cell therapy) may represent an additional or alternative approach to cytokine therapy or allogeneic transplantation. Peripheral blood CD34+ cells could be a useful source of cells for this process provided that collection and ex vivo expansion of hematopoietic stem and progenitor cells could be optimized. Here we investigated whether mesenchymal stem cells could sustain culture of irradiated peripheral blood CD34+ cells. In vitro irradiated (4 Gy 60Co gamma rays) or nonirradiated mobilized peripheral blood CD34+ cells from baboons were cultured for 7 days in a serum-free medium supplemented with stem cell factor+thrombopoietin+interleukin 3+FLT3 ligand (50 ng/ml each) in the presence or absence of mesenchymal stem cells. In contrast to cultures without mesenchymal stem cells, irradiated CD34+ cells cultured with mesenchymal stem cells displayed cell amplification, i.e. CD34+ (4.9-fold), CD34++ (3.8-fold), CD34++/Thy-1+ (8.1-fold), CD41+ (12.4-fold) and MPO+ (50.6-fold), although at lower levels than in nonirradiated CD34+ cells. Fourteen times more clonogenic cells, especially BFU-E, were preserved when irradiated cells were cultured on mesenchymal stem cells. Moreover, we showed that the effect of mesenchymal stem cells is related mainly to the reduction of apoptosis and involves cell-cell contact rather than production of soluble factor(s). This experimental model suggests that mesenchymal stem cells could provide a crucial tool for autologous cell therapy applied to accidentally irradiated victims.
Keywords
Animals, Antigens, CD34/*metabolism, Cell Proliferation/drug effects/radiation effects, Cell Survival/drug effects/radiation effects, Cells, Cultured, Coculture Techniques/*methods, Cytokines/*pharmacology, Hematopoietic Stem Cell Mobilization/methods, Hematopoietic Stem Cells/drug effects/*metabolism/*radiation effects, Humans, Leukocytes/drug effects/metabolism/radiation effects, Mesenchymal Stem Cells/*metabolism, Papio, Radiation Injuries/surgery, Radiation Tolerance/physiology, Stem Cell Transplantation/methods
Pubmed
Create date
02/05/2024 10:41
Last modification date
28/05/2024 7:10
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