Proteomics-based identification of alpha1-antitrypsin and haptoglobin precursors as novel serum markers in infiltrating ductal breast carcinomas.

Details

Serval ID
serval:BIB_7504DDC541F8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Proteomics-based identification of alpha1-antitrypsin and haptoglobin precursors as novel serum markers in infiltrating ductal breast carcinomas.
Journal
Clinica chimica acta; international journal of clinical chemistry
Author(s)
Hamrita B., Chahed K., Trimeche M., Guillier C.L., Hammann P., Chaïeb A., Korbi S., Chouchane L.
ISSN
1873-3492 (Electronic)
ISSN-L
0009-8981
Publication state
Published
Issued date
27/06/2009
Peer-reviewed
Oui
Volume
404
Number
2
Pages
111-118
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The identification of pathological markers of breast cancer for either diagnosis, treatment response or for survival is of critical importance.
Serum protein profiling using 2-DE separations coupled to matrix-assisted laser desorption ionization mass spectrometry has been used to explore protein alterations in patients with infiltrating ductal breast carcinomas (IDCA). Sera from 39 breast cancer patients and 40 healthy controls were selected for screening study using 2-DE combined with MS. The protein expression patterns obtained after the depletion of high abundance proteins was determined by coomassie blue G-250 stain after 2-DE electrophoresis.
Six proteins that expressed differentially in the IDCA group were found. The expression levels of four isoforms corresponding to haptoglobin precursor and two isoforms of alpha1-antitrypsin precursor (alpha1-AT) were upregulated in sera from breast cancer patients. There was an increased expression of both proteins in the sera of patients with various tumor stages (I, II, III) in comparison to healthy women. Applying immunohistochemistry, we further validated alpha1-AT immunoreactivity in 51 formalin-fixed paraffin-embedded sections of breast tumors. Enhanced expression of alpha1-AT like activity has been found in IDCA breast tumors, as well as, in different histological types of breast cancer. No significant association has been found with lymph node occurrence, while in high tumor categories a tendency to an increased expression of alpha1-AT has been found, thereby suggesting a possible role of this protein in tumor growth.
These proteins may constitute new and useful markers of breast cancer that offer a clue to a better understanding of inflammatory pathways and carcinogenesis events linked to breast cancer progression.
Keywords
Adult, Aged, Aged, 80 and over, Amino Acid Sequence, Biomarkers, Tumor/blood, Breast Neoplasms/blood, Breast Neoplasms/pathology, Carcinoma, Ductal/blood, Carcinoma, Ductal/pathology, Electrophoresis, Gel, Two-Dimensional, Female, Haptoglobins/analysis, Humans, Middle Aged, Molecular Sequence Data, Protein Isoforms/blood, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, alpha 1-Antitrypsin/blood
Pubmed
Web of science
Create date
17/10/2023 10:04
Last modification date
20/10/2023 7:10
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