A high resolution LC-MS targeted method for the concomitant analysis of 11 contraceptive progestins and 4 steroids.
Details
Serval ID
serval:BIB_74E38B4C9476
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A high resolution LC-MS targeted method for the concomitant analysis of 11 contraceptive progestins and 4 steroids.
Journal
Journal of pharmaceutical and biomedical analysis
ISSN
1873-264X (Electronic)
ISSN-L
0731-7085
Publication state
Published
Issued date
25/10/2019
Peer-reviewed
Oui
Volume
175
Pages
112756
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
In the context of hormonal contraception and hormone replacement therapy (HRT), many women are exposed to exogenous hormones. Current use of hormonal contraception with combined ethinyl estradiol and different progestins bestows a breast cancer relative risk (RR) of 1.2- while combined HRT has a RR of 2. Although these exposures present an important public health issue, little is known about the effects of individual progestins on the breast and other tissues. Increasing availability of large scale biobanks, high throughput analyses and data management tools enable ever expanding, sophisticated population studies. In order to address the impact of distinct progestins on various health indicators, it is desirable to accurately quantify progestins in clinical samples. Here we have developed and validated a high resolution liquid chromatography mass spectrometry (LC-MS) targeted method for the simultaneous quantification of 11 synthetic progestins widely used in oral contraceptives, gestodene, levonorgestrel, etonogestrel, chlormadinone acetate, cyproterone acetate, drospirenone, desacetyl norgestimate, medroxyprogesterone acetate, norethindrone, dienogest, nomegestrol acetate, and 4 endogenous steroid hormones, progesterone, testosterone, androstenedione, and cortisol in blood samples. This highly specific quantitative analysis with high resolution Orbitrap technology detects and quantifies 15 compounds using their internal standard counterparts in a single 12 min LC-MS run. Sensitivity is attained by the use of the instrument in targeted selected ion monitoring mode. Lower limit of quantitation ranges from 2.4 pg/ml for drospirenone to 78.1 pg/ml for chlormadinone acetate. The method provides comprehensive progestin panel measurements with as little as 50 μl of murine or human plasma.
Keywords
Animals, Chromatography, Liquid/methods, Contraceptive Agents/chemistry, Female, Humans, Mice, Mice, Inbred NOD, Progestins/chemistry, Steroids/chemistry, Tandem Mass Spectrometry/methods, Contraceptives, HRT, Hormones, LC- HRMS, Progestins, Steroids
Pubmed
Web of science
Open Access
Yes
Create date
18/08/2019 15:10
Last modification date
30/01/2020 7:20