Nanog organizes transcription bodies.

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State: Public
Version: Final published version
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Serval ID
serval:BIB_7494A23EDC61
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nanog organizes transcription bodies.
Journal
Current biology
Author(s)
Kuznetsova K., Chabot N.M., Ugolini M., Wu E., Lalit M., Oda H., Sato Y., Kimura H., Jug F., Vastenhouw N.L.
ISSN
1879-0445 (Electronic)
ISSN-L
0960-9822
Publication state
Published
Issued date
09/01/2023
Peer-reviewed
Oui
Volume
33
Number
1
Pages
164-173.e5
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The localization of transcriptional activity in specialized transcription bodies is a hallmark of gene expression in eukaryotic cells. <sup>1</sup> <sup>-</sup> <sup>3</sup> How proteins of the transcriptional machinery come together to form such bodies, however, is unclear. Here, we take advantage of two large, isolated, and long-lived transcription bodies that reproducibly form during early zebrafish embryogenesis to characterize the dynamics of transcription body formation. Once formed, these transcription bodies are enriched for initiating and elongating RNA polymerase II, as well as the transcription factors Nanog and Sox19b. Analyzing the events leading up to transcription, we find that Nanog and Sox19b cluster prior to transcription. The clustering of transcription factors is sequential; Nanog clusters first, and this is required for the clustering of Sox19b and the initiation of transcription. Mutant analysis revealed that both the DNA-binding domain as well as one of the two intrinsically disordered regions of Nanog are required to organize the two bodies of transcriptional activity. Taken together, our data suggest that the clustering of transcription factors dictates the formation of transcription bodies.
Keywords
Animals, Zebrafish/genetics, Zebrafish/metabolism, Nanog Homeobox Protein/genetics, Nanog Homeobox Protein/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Embryonic Development/genetics, Zebrafish Proteins/genetics, Zebrafish Proteins/metabolism, Transcription, Genetic, Homeodomain Proteins/genetics, Homeodomain Proteins/metabolism, SOX Transcription Factors/genetics, SOX Transcription Factors/metabolism, Nanog, RNA polymerase II, nuclear organization, transcription, transcription bodies, transcription factors, zebrafish
Pubmed
Web of science
Create date
03/01/2023 15:09
Last modification date
18/09/2024 7:07
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