Thyroid hormone in biodegradable nerve guides stimulates sciatic nerve regeneration: a potential therapeutic approach for human peripheral nerve injuries.

Details

Serval ID
serval:BIB_748EF6089B77
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Thyroid hormone in biodegradable nerve guides stimulates sciatic nerve regeneration: a potential therapeutic approach for human peripheral nerve injuries.
Journal
Journal of neurotrauma
Author(s)
Barakat-Walter I., Kraftsik R., Krafsik R., Schenker M., Kuntzer T.
ISSN
0897-7151
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
24
Number
3
Pages
567-77
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
It has been already demonstrated that thyroid hormone (T3) is one of the most important stimulating factors in peripheral nerve regeneration. We have recently shown that local administration of T3 in silicon tubes at the level of the transected rat sciatic nerve enhanced axonal regeneration and improved functional recovery. Silicon, however, cannot be used in humans because it causes a chronic inflammatory reaction. Therefore, in order to provide future clinical applications of thyroid hormone in human peripheral nerve lesions, we carried out comparative studies on the regeneration of transected rat sciatic nerve bridged either by biodegradable P(DLLA-(-CL) or by silicon nerve guides, both guides filled with either T3 or phosphate buffer. Our macroscopic observation revealed that 85% of the biodegradable guides allowed the expected regeneration of the transected sciatic nerve. The morphological, morphometric and electrophysiological analysis showed that T3 in biodegradable guides induces a significant increase in the number of myelinated regenerated axons (6862 +/- 1831 in control vs. 11799 +/- 1163 in T3-treated). Also, T3 skewed the diameter of myelinated axons toward larger values than in controls. Moreover, T3 increases the compound muscle action potential amplitude of the flexor and extensor muscles of the treated rats. This T3 stimulation in biodegradable guides was equally well to that obtained by using silicone guides. In conclusion, the administration of T3 in biodegradable guides significantly improves sciatic nerve regeneration, confirming the feasibility of our technique to provide a serious step towards future clinical application of T3 in human peripheral nerve injuries.
Keywords
Absorbable Implants, Animals, Axons, Behavior, Animal, Cell Count, Cell Size, Electrophysiology, Humans, Microscopy, Electron, Nerve Fibers, Myelinated, Nerve Regeneration, Peripheral Nerves, Rats, Sciatic Nerve, Silicon, Thyroid Hormones, Triiodothyronine
Pubmed
Web of science
Create date
25/01/2008 12:44
Last modification date
20/08/2019 14:32
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