Soybean (Glycine max. L.) and bacteroid glyoxylate cycle activities during nodular senescence

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Version: author
Serval ID
serval:BIB_7457CD02C280
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Soybean (Glycine max. L.) and bacteroid glyoxylate cycle activities during nodular senescence
Journal
Journal of Plant Physiology
Author(s)
Fargeix C., Gindro K., Widmer F.
ISSN
0176-1617
Publication state
Published
Issued date
2004
Peer-reviewed
Oui
Volume
161
Number
2
Pages
183-190
Language
english
Abstract
Soybean (Glycine max. L.) nodular senescence results in the dismantling of the peribacteroid membrane (PBM) and in an increase of soybean isocitrate lyase (ICL; EC 4.1.3.1) and malate synthase (MS; EC 4.1.3.2) mRNA and protein levels. This suggests that in senescing soybean nodular cells, the specific glyoxylate cycle enzyme activities might be induced to reallocate carbon obtained from the PBM degradation. In order to evaluate as well the carbon metabolism of the nitrogen-fixing Bradyrhizobium japonicum endosymbiotic bacteroids during nodular senescence, their glyoxylate cycle activities were also investigated. To this end, partial DNA sequences were isolated from their icl and ms genes, but the corresponding mRNAs were not detected in the microorganisms. It was also observed that the bacteroid ICL and MS activities were negligible during nodular senescence. This suggests that glyoxylate cycle activities are not reinitiated in the bacteroids under these physiological conditions. In case the microorganisms nevertheless feed on the PBM degradation products, this might occur via the citric acid cycle exclusively.
Keywords
Bradyrhizobium japonicum, Glycine max. L., glyoxylate cycle, isocitrate lyase malate, synthase, peribacteroid membrane, senescence, soybean, DARK-INDUCED SENESCENCE, ROOT-NODULES, ENZYME-ACTIVITIES, ISOCITRATE LYASE, LEAF SENESCENCE, GENE-EXPRESSION, PATHOGENIC ATTACK, BARLEY LEAVES, ACID CYCLE, COTYLEDONS
Pubmed
Web of science
Create date
13/08/2015 7:54
Last modification date
20/08/2019 14:32
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